From cloning and transformation through fermentation, downstream purification and final product testing, microbial protein expression and  production requires:

  • host cell lines
  • chemically defined media and vectors
  • high capacity, high resolution, salt-tolerant resins for polish chromatography
  • validated and automated rapid contaminant and impurity tests

 

Through brands you trust — POROS® chromatography resins, and Applied Biosystems® SEQ rapid contaminant tests — Life Technologies is helping to set new manufacturing standards in product quality, safety and fast-to-market efficiency for  microbial expression and production. 

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Step-by-Step Guide to Microbial Protein Production Products

Sample solutions differ widely between various applications. To enable downstream success, check expression levels during the cloning and transformation process prior to initiating large scale fermentation.

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Recommended Products

Partner with Life Technologies to develop custom media that meets the specific needs of varied growth and induction condition requirements. Optimize yield, and save time, by monitoring expression at many points during fermentation and validating cell paste prior to large scale purification release.

POROS® Chromatography resins are today’s best performing chromatographic media for process-scale bioseparations. POROS® resins are available in a range of selections designed especially for today’s recombinant protein and other biological purification challenges.

Quickly help verify purity and identify potential contamination with thousands of bacterial and fungal species in the final product using the MicroSEQ® Microbial Identification System.

Bioproduction Resources

Have questions about Gibco® media, POROS® chromatography resins, Applied Biosystems® SEQ rapid contaminant tests, or our Cell Therapy products?  Click below to access product brochures, training videos, mobile apps, and more.

 

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