POROS® A, G, and CaptureSelect™ HPLC columns incorporate the advantages of high-speed POROS® chromatography resins with the unique selectivity of Protein A, Protein G, and CaptureSelect™ ligands (Figure 1). The combination allows 3-minute quantitation of immunoglobulins, fusion proteins, Fabs, and biospecific antibodies.

  • Broad utility—POROS® A, G, and CaptureSelect™ ligands address most biological drug classes
  • Rapid—POROS® resins offer fast quantitation or purification of immunoglobulins and fusion proteins
  • Hands-off operation—HPLC method can be automated for streamlined operation
  • Robust—deliver high-performance purification over hundreds of chromatography cycles
Enlarge Image

New POROS® Ligands Provide Alternative Specificities

High Speed with High Specificity

The combination of Protein A, Protein G, and CaptureSelect™ affinity ligands when combined with POROS® 20 µm resin provides a high flow rate, high-performance chromatography solution for the rapid quantitation and small-scale purification of:

  • IgG—all subclasses, specific for human
  • IgM—specific for human, mouse, or rat
  • Kappa light chain—specific for human Igs and Fab fragments with a kappa light chain
  • Lambda light chain—specific for human Igs and Fab fragments with a lambda light chain
  • Human serum albumin
  • Fusion proteins, Fc, or human serum albumin
  • Follicle stimulating hormone (FSH)—binding only the intact form of FSH
Find data about each affinity ligand

 

An example of the chromatographic performance of POROS® CaptureSelect™ LC-Kappa with five unique kappa Fab fragments is shown in Figure 2a. The associated standard curves, Figure 2b, exhibit excellent linearity.

Check out the CaptureSelect™ Technology

Enlarge Image
Figure 2a POROS® resins exhibit high speed and efficiency, regardless of kappa light chain. These overlaid chromatograms of 5 kappa light chains run on a POROS® LC Kappa column illustrate the speed and efficiency of the analysis.
Enlarge Image
Figure 2b POROS® kappa standard curves exhibit good linear range (8x) and linear correlation coefficients (R2 range: 1 to 0.9991).

Chromatography details: POROS® CaptureSelect™ LC-Kappa column (Cat. No. 4469149, 2.1 x 30 mm, PEEK™ polymer); System: Agilent 1200, flow rate 2 mL/min; Sample injection: 50 μL of purified kappa light chain at a 0.5 mg/mL; Equilibration buffer: phosphate buffer, 150 mM NaCl pH 7.5; Elution buffer: 12 mM HCl, 150 mM NaCl pH 1.9; UV detection: 280 nm.

POROS® Affinity HPLC Columns

POROS® A, G, and ligands are available in convenient prepacked HPLC columns. The column range addresses analytical and preparative requirements for the quantitation and purification of immunoglobulins or Fc fusion or HSA fusion proteins.

POROS® CaptureSelect® HSA Chromatography Columns Products

Sku Name Size Price Qty
4469151 POROS® CaptureSelect® HSA Affinity Column, 2.1 x 30 mm 1 column USD 1,830.00
4469165 POROS® CaptureSelect® HSA Affinity Column, 4.6 x 50 mm 1 column USD 1,882.00
4469170 POROS® CaptureSelect® HSA Affinity Column, 4.6 x 100 mm 1 column USD 2,290.00
4469175 POROS® CaptureSelect® HSA Affinity Column, 10 x 100 mm 1 column USD 7,930.00

POROS® CaptureSelect® IgG Fc Chromatography Columns Product List

Sku Name Size Price Qty
4469148 POROS® CaptureSelect® IgG Fc Affinity Column, 2.1 x 30 mm 1 column USD 1,830.00
4469153 POROS® CaptureSelect® IgG Fc Affinity Column, 4.6 x 50 mm 1 column USD 1,882.00
4469166 POROS® CaptureSelect® IgG Fc Affinity Column, 4.6 x 100 mm 1 column USD 2,290.00
4469171 POROS® CaptureSelect® IgG Fc Affinity Column, 10 x 100 mm 1 column USD 7,930.00

POROS® CaptureSelect® IgM Chromatography Columns Product List

Sku Name Size Price Qty
4469152 POROS® CaptureSelect® IgM Affinity Column, 2.1 x 30 mm 1 column USD 1,830.00
4469164 POROS® CaptureSelect® IgM Affinity Column, 4.6 x 50 mm 1 column USD 1,882.00
4469169 POROS® CaptureSelect® IgM Affinity Column, 4.6 x 100 mm 1 column USD 2,290.00
4469174 POROS® CaptureSelect® IgM Affinity Column, 10 x 100 mm 1 column USD 7,930.00

POROS® CaptureSelect® LC Kappa Chromatography Columns Product List

Sku Name Size Price Qty
4469149 POROS® CaptureSelect® LC Kappa Affinity Column, 2.1 x 30 mm 1 column USD 1,830.00
4469162 POROS® CaptureSelect® LC Kappa Affinity Column, 4.6 x 50 mm 1 column USD 1,882.00
4469167 POROS® CaptureSelect® LC Kappa Affinity Column, 4.6 x 100 mm 1 column USD 2,290.00
4469172 POROS® CaptureSelect® LC Kappa Affinity Column, 10 x 100 mm 1 column USD 7,930.00

POROS® CaptureSelect® LC-Lambda Product List

Sku Name Size Price Qty
4469150 POROS® CaptureSelect® LC Lambda Affinity Column, 2.1 x 30 mm 1 column USD 1,830.00
4469163 POROS® CaptureSelect® LC Lambda Affinity Column, 4.6 x 50 mm 1 column USD 1,882.00
4469168 POROS® CaptureSelect® LC Lambda Affinity Column, 4.6 x 100 mm 1 column USD 2,290.00
4469173 POROS® CaptureSelect® LC Lambda Affinity Column, 10 x 100 mm 1 column USD 7,930.00

POROS® CaptureSelect® FSH Chromatography Columns Product List

Sku Name Size Price Qty
4481822 POROS® CaptureSelect® FSH Affinity Column, 2.1 x 30 mm 1 column USD 1,830.00
4481824 POROS® CaptureSelect® FSH Affinity Column, 4.6 x 50 mm 1 column USD 1,882.00
4481828 POROS® CaptureSelect® FSH Affinity Column, 10 x 100 mm 1 column USD 7,930.00
4481826 POROS® CaptureSelect® FSH Affinity Column, 4.6 x 100 mm 1 column USD 2,290.00

POROS® CaptureSelect® GCSF Chromatography Columns Product List

Sku Name Size Price Qty
4485157 POROS® CaptureSelect® GCSF Affinity Column, 2.1 x 30 mm 1 column USD 1,800.00
4485164 POROS® CaptureSelect® GCSF Affinity Column, 4.6 x 50 mm 1 column USD 1,850.00
4485168 POROS® CaptureSelect® GCSF Affinity Column, 4.6 x 100 mm 1 column USD 2,250.00
4485172 POROS® CaptureSelect® GCSF Affinity Column, 10 x 100 mm 1 column USD 7,800.00

POROS® CaptureSelect® hGH Chromatography Columns Product List

Sku Name Size Price Qty
4485161 POROS® CaptureSelect® hGH Affinity Column, 2.1 x 30 mm 1 column USD 1,800.00
4485165 POROS® CaptureSelect® hGH Affinity Column, 4.6 x 50 mm 1 column USD 1,850.00
4485169 POROS® CaptureSelect® hGH Affinity Column, 4.6 x 100 mm 1 column USD 2,250.00
4485173 POROS® CaptureSelect® hGH Affinity Column, 10 x 100 mm 1 column USD 7,800.00

POROS® CaptureSelect® IgA Chromatography Columns Product List

Sku Name Size Price Qty
4485162 POROS® CaptureSelect® IgA Affinity Column, 2.1 x 30 mm 1 column USD 1,800.00
4485166 POROS® CaptureSelect® IgA Affinity Column, 4.6 x 50 mm 1 column USD 1,850.00
4485170 POROS® CaptureSelect® IgA Affinity Column, 4.6 x 100 mm 1 column USD 2,250.00
4485174 POROS® CaptureSelect® IgA Affinity Column, 10 x 100 mm 1 column USD 7,800.00

Learn more about other related Life Technologies products:

Video: What's in a Resin? Find out why the POROS resin is the best chromatography media available today
View it now >

POROS® XS Sample!

Request a sample of our high capacity, high resolution 50uM cation chromatography resin for evaluation.

Request Sample

Webinar: Please join us for our free webinar on a new approach to anion exchange
View it now >

Column Calculator

In a few mouse clicks, calculate the volumetric or linear flow rate of your column with our simple desktop calculator widget.

Download & details

Connect with Bioproduction

Like us on Facebook
Watch us on YouTube