Large-Scale Process Chromatography
When you need high performance and high throughput on a large scale, POROS® bulk chromatography resins are the industry standard for process-scale bioseparations.
Utilized in multiple processes, these breakthrough resins combine optimal particle size and pore structure with a rigid backbone and proprietary chemistries. POROS® 50 µm resins demonstrate exceptionally high dynamic binding capacity, capture efficiency, and resolution over a range of flow rates.
POROS® products are manufactured in a state-of-the-art chromatography resin manufacturing facility and are tested using the highest-quality standards to support use in regulated biotherapeutic markets.
CaptureSelect™ affinity resins offer superior selectivity resulting in higher yields and improved purity in the initial capture step. We are developing numerous products for large scale purification of various biologics such as antibodies and antibody fragments, fusion proteins, hormones and viruses.
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Benefits of a Revised Approach to Anion Exchange Flow-Through Polish Chromatography.
A high-performance anion exchange resin performs well compared with membranes. In addition, the resin offers greater flexibility and cost savings
The new POROS® XS Cation Exchange Resin delivers:
- Dynamic binding capacity for proteins, >100 mg/mL at 5% breakthrough
- High resolution using optimized 50 μm particle size for improved impurity clearance
- Robust salt tolerance that maintains dynamic binding capacity to 150 mM NaCl (15 mS/cm)
Many products incorporating CaptureSelect™ technology have already been developed and are used for clinical and commercial manufacturing of a variety of Biologicals. To support these products Life Technologies has developed product specific Leakage ELISAs that can be used for routine quality analysis. Below a list of affinity resins and their corresponding Leakage ELISAs.
|Purification Target||Chromatography Resin||Leakage ELISA|
|Human IgG||IgSelect*||IgSelect leakage assay|
|Fab Kappa||KappaSelect*||KappaSelect leakage assay|
|Adeno-Associated Virus||AVB sepharose HP*||AVB Sepharose HP leakage assay|
|Recombinant Factor VIII||VIIISelect*||VIIISelect leakage assay|
|Human Alpha-1 Antitrypsin (hAAT)||Alpha-1 Antitrypsin Select*||Alpha-1 Antitrypsin leakage assay|
|Factor VII||VIISelect*||VIISelect leakage assay|
|Fab lambda||LambdaFabSelect*||LambdaFabSelect leakage assay|
|Factor IX||IXSelect*||IXSelect leakage assay|
|Granulocyte colony-stimulating factor||GCSFSelect*||GCSFSelect leakage assay|
* Marketed and sold by GE Healthcare Life Sciences.
POROS® XS 50 µm Bulk Resin
A next-generation strong cation exchange resin that delivers a combination of high capacity, high resolution, and high salt tolerance for greater process flexibility and performance.
POROS® HS 50 µm Bulk Resin
A strong cation exchange resin offering high resolution for the removal of process contaminants and product impurities in downstream polish applications,
POROS® HQ 50 µm Bulk Resin
A strong anion exchange resin providing high resolution with high protein binding capacity and efficiency
POROS® D 50 µm Bulk Resin
A weak anion exchange resin providing a high throughput alternative to traditional DEAE resins
POROS® PI 50 µm Bulk Resin
A weak anion exchange resin deliving unique selectivity due to its polyethyline imine functionality
CaptureSelect™ Bioprocess Products
CaptureSelect™ affinity ligands, based on Camelid-derived single domain antibody fragments, are available for use in large-scale manufacturing processes. Paired leakage assays offer additional tools for process development or for routine quality control processes.
POROS® MabCapture™ A Resin
A high performance Protein A chromatography resin technology combining improvements made in bead and chemistry technology to provide high dynamic binding capacities and excellent capture efficiencies at high linear velocities
POROS® Heparin 50 µm Bulk Resin
Heparin affinity media for process scale separation of biomolecules with sepcific Heparin binding affinity, such as growth factors and other serum components, including coagulation factors and nucleotide binding proteins.
POROS® R1 50 µm Bulk Resin
A reversed-Phase Resin for large scale downstream bioprocessing. POROS® R1 is a 4000 angstrom pore size Poly (Styrene-Divinylbenzene) particle, suitable for the reversed-phase separation of biomolecules.
POROS® R2 50 µm Bulk Resin
A reversed-Phase Resin for large scale downstream bioprocessing. POROS® R2 is a 2000 angstrom pore size Poly (Styrene-Divinylbenzene) particle, suitable for the reversed-phase separation of biomolecules.