Whole exome sequencing in cancer research
Exome sequencing is a fast and affordable alternative to using whole genome sequencing for your research, allowing you to identify somatic and germline genetic variants within coding regions of genes without the additional expense and data analysis challenges associated with whole genome sequencing.
Whole exome sequencing
Most of the DNA sequence variants that lead to alterations in protein function are located in exons, which encompass approximately 1% of the genome. By specifically targeting exon regions, whole exome sequencing is a simple and efficient approach to identify rare mutations and help discover new biomarkers.
With the fastest run times of any benchtop next-generation sequencer, at the most affordable price, the Ion Proton™ Sequencer provides a simple, scalable, and affordable solution for whole exome sequencing in cancer research. Combined with the Ion Chef™ System* for automated template preparation and the integrated Ion Reporter™ workflows for data analysis, the Ion Proton™ System is an excellent choice for biomarker discovery.
Hereditary cancer research studies
The Ion AmpliSeq™ Exome Kit enables the simplest and fastest sample preparation workflow for exome sequencing available today. Designed for sequencing DNA from fresh or frozen samples, this solution is ideal for identifying germline variants in familial research studies. Simplifying trio analysis, father/mother/proband samples can be easily sequenced on a single chip.
The TargetSeq™ Exome Kit enables high on-target sequencing reads with uniform coverage from both archived FFPE samples and fresh-frozen tissues. Ideal for exome sequencing of tumor samples, normal/tumor pairs, and primary/metastatic tumor pairs, this easy-to-use sequencing kit enables consistent and reliable research results across multiple tissue sources.
“This system (Ion Ampliseq™ Exome Solution) gives us great flexibility compared to other systems. We can take on multiple projects of various size, running just one or up to 24 samples in a week, and turn them around in just two days without the need to batch to keep our costs down. This is especially important when dealing with urgent samples requiring fast turnaround time, such as clinical research specimens.“ Jeremy Stuart
Vice President of Genomic Services
Greenville, South Carolina, USA
Dr. Joe Boland
Frederick National Laboratory for Cancer Research SAIC-Frederick
Dr. Joe Boland presents his experience using the PGM™ and Ion Proton™ sequencers for whole exome sequencing and targeted sequencing in cancer genome research.
* The content provided herein may relate to products that have not been officially released and is subject to change without notice.
For Research Use Only. Not for use in diagnostic procedures.