Cell Isolation With Dynabeads® Technology
Gentle and tube-based magnetic separation with Dynabeads® is the technology of choice when you want to isolate high yields of pure, viable and functional cells. Your cells are not exposed to the stress of going through a dense column, and there's no risk of your results being affected by the isolation method.
You can easily isolate any cell subset using Dynabeads® and a DynaMag™ magnet. Your specific product of choice will depend on the species and the isolation strategy:
Selection Guides for Cell Isolation Products:
Human Cell Separation
Mouse Cell Separation
Separation of Other Cell Types
Cell Separation Products
|Human Cell Separation||Mouse Cell Separation||Secondary Cell Separation|
Positive Cell Isolation
In positive isolation, Dynabeads® bind the target cells and the bead/cell complexes are pulled to the magnet. The supernatant is discarded, leaving only your pure target cells,
Directly after cell separation, the beads can easily be released and removed using the innovative Dynabeads® FlowComp™ technology.
Other Dynabeads® release systems include the CELLection™, and the DETACHaBEAD™ technologies.
click image to see FlowComp™ workflow
Negative Cell Isolation
In negative isolation, Dynabeads® are used to deplete all the unwanted cells using the supplied antibody mix. Your bead-free and untouched target cells are left in the sample, and can be directly analyzed in a flow cytometer and used in any functional assay.
- Learn More about Negative Cell Separation
Should you want to refine for a particular cell type, you can of course add extra antibodies to the antibody mix. With the secondary-coated Dynabeads® you can create your own negative isolation kit for isolation of any cells from any species.
In depletion, cell-specific Dynabeads® are used to remove specific unwanted cell types from a mixed cell sample with >99% efficiency. Recover the depleted supernatant - still containing all your wanted cells - for downstream assays. Click for more info on: Dynabeads® for human cell depletion and mouse cell depletion.
You can also use the positively targeted and bead-bound cells for downstream molecular applications that do not require release of cells.
- Start your T cell research right (brochure)
- Dynabeads® FlowComp (brochure)
- Human and mouse Treg cell isolations (brochure)
- Treg isolation & expansion (poster)
- 1-step NK cell isolation (poster)
- When viability matters - Dynabeads® for gentle cell isolation (flyer)
- Reference in JCB: Using Dynabeads® M-270 Streptavidin for ChIP and QPCR (article )