Phalloidin Conjugates For Staining Actin
We provide a number of Molecular Probes® fluorescent and biotinylated derivatives of phalloidin that deliver significant advantages over antibodies for actin labeling. The binding properties of phalloidin conjugates do not change appreciably with actin from different species, including plants and animals. In addition, they exhibit negligible nonspecific staining, so the contrast between stained and unstained areas is high.
See additional products for cytoskeleton staining
Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin in fixed cells, permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates have similar affinity for both large and small filaments and bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and nonmuscle cells; they reportedly do not bind to monomeric G-actin, unlike some antibodies against actin.
Alexa Fluor® phalloidin conjugates
Fluorescent conjugates of phalloidin using the Molecular Probes® Alexa Fluor® dyes are widely used F-actin stains, with color choices across the full spectral range. Alexa Fluor® phalloidin conjugates provide researchers with fluorescent probes that are designed to be superior in brightness and photostability to all other spectrally similar conjugates tested.
Fixed, permeabilized bovine pulmonary artery endothelial cells visualized using components of the SelectFX® Nuclear Labeling Kit and Alexa Fluor® phalloidin conjugates. Nuclei and F-actin were stained, respectively, with (top left) DAPI and Alexa Fluor® 680 phalloidin, (top right) SYTOX® Green dye and Alexa Fluor® 568 phalloidin, (bottom left) 7-AAD and Alexa Fluor® 488 phalloidin, and (bottom right) TO-PRO®-3 dye and Alexa Fluor® 350 phalloidin.
Spectral characteristics of Molecular Probes® phalloidin probes
|Actin-selective probe||Ex/Em*||~ MW||Cat. No.|
|Alexa Fluor® 350 Phalloidin||346/442 †||1100||A22281|
|Fluorescein phalloidin||496/516 †||1175||F432|
|Alexa Fluor® 488 Phalloidin||496/519 †||1180||A12379|
|Oregon Green® 488 phalloidin||496/520 †||1180||O7466|
|BODIPY® FL Phallacidin||505/512||1125||B607|
|Oregon Green® 514 phalloidin||511/528 †||1281||O7465|
|Rhodamine phalloidin||540/565 †||1250||R415|
|Alexa Fluor® 555 Phalloidin||555/565 †||1910||A34055|
|BODIPY® 558/568 phalloidin||558/569||1115||B3475|
|Alexa Fluor® 594 Phalloidin||590/617 †||1620||A12381|
|Texas Red®-X phalloidin||591/608 †||1490||T7471|
|BODIPY® 650/665 Phalloidin||647/661||1200||B12382|
|Alexa Fluor® 647 Phalloidin||650/665 †||1950||A22287|
|Alexa Fluor® 680 Phalloidin||679/702 †||1850||A22286|
|* Excitation (Ex) and emission (Em) maxima, in nm. Spectra of phallotoxins are either in aqueous buffer, pH 7–9 (denoted †) or in methanol.|