Neuronal tracing

Neuronal morphology and connectivity are key components in neuronal function. Molecular Probes® labeling and detection tools include a diverse selection of probes for dissecting neural networks and their functions, with an extensive palette of fluorescent colors for each class of probes.

Fluorescent tracers have the advantage of being imaged directly, or they can serve as haptens for antibodies or streptavidin dye conjugates used to amplify the signal.

The technologies used to trace neural networks can be divided into those for tracing single cells and those for monitoring cell populations. While most fluorescent probes are effective in experiments involving fresh tissue sections and cultured cells, there are special requirements for fixed tissues and in vivo imaging.

Hydrazides & Biocytins

hydrazides & biocytins for neuron staining  

Loaded by microinjection or iotophoresis, these fixable and photostable probes are typically employed to trace neuronal projections and investigate gap junctions.

Cell Membrane Staining

cell membrane staining  

By lateral diffusion within the plasma membrane, lipophilic tracers label cell membranes without affecting cell viability. Or use them to trace neuronal projections in fixed tissue sections.

Dextran Conjugates

dextran conjugates for neuron staining   Loaded by microinjection or iontophoresis, dextrans trace neuronal projections and can be used to investigate gap junctions.

 

Protein Conjugates

Protein conjugates for neuron staining   Useful for live or fixed cells and introduced by microinjection or iontophoresis, this range of fixable, site-selective fluorescent probes provides choices for axonal membrane staining and neuronal sybtype differentiation.