Dextran conjugates for neuronal staining

Typically employed for tissue sections, in vivo neurons, and cultured cells, 3,000 MW dextrans are used to trace fine neuron projections and investigate gap junctions; 10,000 MW dextrans have slower distribution and longer cellular retention and do not cross gap junctions. The staining pattern produced by most of these labeled dextran products is lysine fixable.


Select dextran conjugates for neuron staining

Dextran Conjugates for Neuron Staining

  • 3,000 and 10,000 MW
  • Direct fluorescence or secondary detection
  • Load by microinjection or iontophoresis
  • Fixable and photostable options

Fluorescent and biotinylated dextrans are routinely used to trace neuronal projections. 3,000 MW dextrans are rapidly dispersed into fine cellular projections and can be used to investigate gap junctions. By contrast, the 10,000 MW dextrans are retained within the cell and do not cross gap junctions, and they have slower distribution but longer cellular retention.

Fluorecent dextrans can be imaged directly. Biotinylated dextrans are suitable for secondary detection and signal amplification. These are offered in a range of fluorescent colors, and most conjugates are lysine-fixable for use in conjunction with antibody labeling. The 10,000 MW AlexaFluor® conjugates are generally brighter and more photostable.

 Dextran conjugates
Secondary motor neurons in a spinal cord whole mount of a male western mosquitofish labeled with lysine-fixable 3000 MW Texas Red® dextran.

Selection Guides for Dextran Conjugates

Properties

 
3,000 MW Dextran
3,000 MW Dextran, Fluorophore, Biotin
ReadoutDirect fluorescence imaging of cellsDirect fluorescence imaging of cells; biotins easily accept secondary detection to amplify weak signals
CarrierDextran, 3,000 MWDextran, biotin, 3,000 MW
Tissue sectionsYes
In vivo neuronsYes
Fixed tissue sectionsNo
Cultured cellsYes
Cell loadingMicroinjection
Staining modeEndogenous
Best forSingle cells
TransportBidirectional
Synaptic transferNo
Gap junction transferYes
BibliographyCitationsCitations

 

Selection Guide—Lysine-Fixable Dextrans

 
Standard filter set(s)
Fluorophore/conjugate
Ex/Em (nm)
Photobleaching resistance
Quantity
Cat. No.
Dextran, Biotin, 3,000 MWNABiotinNANA10 mgD7135
Dextran, Fluorescein and Biotin, 3,000 MWFITCFluorescein494/5182 stars5 mgD7156
Dextran, Tetramethylrhodamine and Biotin, 3,000 MWTAMRATAMRA555/5802 stars5 mgD7162
Dextran, Fluorescein, 3,000 MWFITCFluorescein494/5212 stars10 mgD3306
Dextran, Tetramethylrhodamine, 3,000 MWTAMRATAMRA555/5802 stars10 mgD3308
Dextran, Texas Red®, 3,000 MWTexas Red®Texas Red®595/6152 stars10 mgD3328

 

Selection Guide—Non-Fixable Dextrans

 
Standard filter set(s)
Fluorophore/conjugate
Ex/Em (nm)
Photobleaching resistance
Quantity
Cat. No.
Dextran, Rhodamine Green™, 3,000 MWFITCRhodamine Green™502/5273 stars5 mgD7163
Dextran, Tetramethylrhodamine, 3,000 MWTAMRATAMRA555/5802 stars10 mgD3307
Dextran, Texas Red®, 3,000 MWTexas Red®Texas Red®595/6152 stars10 mgD3329

Properties

 
10,000 MW Dextran, Alexa Fluor®
10,000 MW Dextran, Fluorophore, Biotin
ReadoutDirect fluorescence imaging of cellsDirect fluorescence imaging of cells; biotins easily accept secondary detection to amplify weak signals
CarrierDextran, 10,000 MWDextran, biotin 10,000 MW
Tissue sectionsYes
In vivo neuronsYes
Fixed tissue sectionsNo
Cultured cellsYes
Cell loadingMicroinjection
Staining modeEndogenous
Best forSingle cells
FixableLysine-fixable
TransportBidirectional
Photobleaching resistance5 stars2 stars
Synaptic transferNo
Gap junction transferNo
BibliographyCitationsCitations

 

Selection Guide

 
Standard filter set(s)
Fluorophore/conjugate
Ex/Em (nm)
Quantity
Cat. No.
Dextran, Alexa Fluor® 488, 10,000 MWFITCAlexa Fluor® 488495/5195 mgD22910
Dextran, Alexa Fluor® 546, 10,000 MWTRITCAlexa Fluor® 546556/5735 mgD22911
Dextran, Alexa Fluor® 555, 10,000 MWAlexa Fluor® 555555/5655 mgD34679
Dextran, Alexa Fluor® 568, 10,000 MWRhodamineAlexa Fluor® 568578/6035mgD22912
Dextran, Alexa Fluor® 594, 10,000 MWTexas Red®Alexa Fluor® 594590/6175 mgD22913
Dextran, Alexa Fluor® 647, 10,000 MWCy®5Alexa Fluor® 647650/6685 mgD22914
Dextran, Alexa Fluor® 680, 10,000 MWCy®5.5Alexa Fluor® 680679/7025 mgD34680
Dextran, Tetramethylrhodamine and Biotin, 10,000 MWTAMRATAMRA555/58010 mgD3312
Dextran, Fluorescein and Biotin, 10,000 MWFITCFluorescein494/51810 mgD7178