Many different approaches are used in the study of programmed cell death, or apoptosis. Cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach for accurate assessment. Understanding the mechanisms of cell death and survival can represent a critical aspect of toxicological profiling and drug discovery.

Life Technologies offers a wide array of apoptosis assays for measuring multiple components on a choice of assay platforms:

  • Caspase activity assays
  • DNA fragmentation and morphology
  • Annexin V staining
  • Membrane potential and other mitochondrial assays

Also see flow cytometry assays for apoptosis

Caspase Activity Assays

Caspase Activity Assays Caspase assays are a distinctive feature of early stage apoptosis.
  • Real-time no wash assay for caspase activity
  • Multi-parameter assays for live and dead cells
  • Antibodies for immunodetection of apoptosis

DNA Fragmentation and Morphology

DNA Fragmentation and Morphology DNA fragmentation and nuclear changes characterize late apoptosis.
  • TUNEL assays formatted for imaging and HCS
  • DNA stain in ready-to-use format
  • Compatible with multiplexed staining procedures

Annexin V Staining

Annexin V Staining   Annexin V staining is used to identify intermediate apoptosis stages.
  • Alexa Fluor® dyes for more sensitive assays
  • Multiple conjugates for multiplexing flexibility
  • Simple protocol and rapid assays

Membrane Potential and Other Mitochondrial Assays

Membrane Potential and Other Mitochondrial Assays   Mitochondrial changes occur early in apoptosis.
  • Real-time assays in live cells
  • Choice of physiological measurements
  • Fixable probes for multiplexing with immunodetection