In normal viable cells, phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane. However, in the intermediate stages of apoptosis, PS is translocated from the inner to the outer leaflet of the membrane, exposing PS to the external cellular environment where it can be detected. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine.

Select annexin V staining products

Also see annexin v conjugates for flow cytometry

Apoptosis Detection with Annexin V Conjugates

  • Alexa Fluor® dyes for more sensitive assays
  • Multiple conjugates for multiplexing flexibility
  • Simple protocol and rapid assays

Life Technologies is collaborating with Nexins Research BV—the original developer of fluorescent phosphatidylserine-binding proteins—to provide what we feel are the best and brightest annexin V conjugates available. These conjugates are very useful for fluorescent imaging as well as for flow cytometry and can be used in combination with other dyes, including nucleic acid stains and immunofluorescence methods to accurately assess mixed populations of apoptotic and nonapoptotic cells.

 Apoptotis detection with Alexa Fluor® 488 Annexin V

Early-stage apoptotis detection in Jurkat human T cell leukemia cells with Alexa Fluor® 488 Annexin V.

Selection Guide for Annexin V Staining Products

The externalization of phosphatidylserine is one of the leading indicators of apoptosis. Annexin V conjugated to Alexa Fluor® dyes provides the brightest probes for detecting phosphatidylserine in live or fixed suspension cells with a broad wavelength selection for multiplexing.

 
Readout Cells expressing external phosphotidylserine generate increased fluorescent signal
Conjugated dye Alexa Fluor® 350 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 680
Common filter sets DAPI FITC TRITC Rhodamine Texas Red® Cy®5 Cy®5.5
Ex/Em (nm) 346/442 495/519 555/565 578/603 590/617 650/668 679/702
Photostability three stars five stars
Signal-to-noise ratio three stars five stars
Bibliography Citations
Fluorescence microscope
Yes
HCS
No
HTS
No
Multiplexing Bright signals and broad wavelength range support easy multiplexing
Live cells Yes
Fixed cells No
Fixable Yes
Format 500 µL,
100 assays
500 µL,
100 assays
500 µL,
100 assays
500 µL,
100 assays
500 µL,
100 assays
500 µL,
100 assays
500 µL,
100 assays
Cat. No. A23202 A13201 A35108 A13202 A13203 A23204 A35109