The later stages of apoptosis are characterized by changes in nuclear morphology, including DNA fragmentation, chromatin condensation, degradation of nuclear envelope, nuclear blebbing, and DNA strand breaks. Life Technologies offers a variety of reagents for analyzing nuclear changes during apoptosis; many are compatible with reagents and procedures used to detect additional apoptotic parameters.

Select nuclear apoptosis assays

Nuclear Apoptosis Assays

  • TUNEL assays formatted for imaging and HCS
  • DNA stain in ready-to-use format
  • Compatible with multiplexed staining procedures

Cells undergoing apoptosis display an increase in nuclear chromatin condensation, which is visible using NucBlue® Live Cell Stain. Staining works for live or fixed cells and is readily combined with other detection procedures.

DNA fragmentation that occurs during apoptosis produces DNA strand breaks, which can be analyzed using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end-labeling) assays. The Click-iT® TUNEL assay is a two-color assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells.

 Endothelial cells stained with NucBlue® Live ReadyProbes™ Reagent.

Endothelial cells stained with NucBlue® Live ReadyProbes™ Reagent.

Selection Guide for Nuclear Apoptosis Assays

Changes in DNA morphology such as nuclear condensation or DNA fragmentation are efficient indicators of apoptosis.

 
ReadoutClick-iT® TUNEL Assays for DNA fragmentation provide efficient discrimination of even high levels of apoptosis in a cell population.Simple indicator of nuclear condensation that is easily multiplexed with other apoptosis markers.
Conjugated dyeAlexa Fluor® 488Alexa Fluor® 594Alexa Fluor® 647Hoechst 33342
Standard filter setFITCTexas Red®Cy®5DAPI
Ex/Em (nm)495/519590/615650/670350/461
BibliographyCitations  Citations
Fluorescence microscopyYesYes
HCSYesYes
HTSNoNo
MultiplexingYesYes
Live cellsNoYes
Fixed cellsMild fixation and permeabilization requiredYes
FixableYesYes
Format1 kit (1 microplate or 50 coverslips)1 kit
Cat. No.C10245C10246C10247R37605