The parameters that define cell viability in a particular experiment can be as diverse as the redox potential of the cell population, the integrity of cell membranes, or the activity of cellular enzymes such as esterases. Each assay provides a different snapshot of cell health, and can individually or together form the basis of an assay for cell viability, cytotoxicity, or drug efficacy with several integrated components.

Specially designed cell viability indicators have been developed for sensing the different characteristics and providing a visual readout of cell health using a fluorescence microscope, microplate reader, or flow cytometer. All indicators have positive and negative attributes; however, their sensitivity, reliability, and compatibility with relevant cell lines are important factors in determining utility.

Also see cell viability assays using flow cytometry

Microplate Assays for Cell Viability

Microplate assays for cell liability   Population-based assays for measuring cell viability.
  • Cellular reduction potential
  • Multi-parameter assays for live and dead cells

Imaging Microbial Viability

Microbial viability   Imaging assays optimized for use with bacteria and yeast.
  • Imaging bacteria and yeast
  • Imaging biofilms

Imaging Mammalian Cell Viability

Mammalian cell viability   Assays for measuring cell viability using imaging.
  • High-content, multi-parameter imaging assays
  • Scalable from single slides to microplates

Other Imaging Assays

Membrane Potential and Other Mitochondrial Assays   Related assays often used to assess viability and cytotoxicity.