Image-iT™ FX Kits
All-in-One Kits for Fluorescence Imaging
The Image-iT™ FX Kits provide all of the dyes and reagents you need for optimal immunofluorescence imaging of fixed cells and tissue sections. Each kit includes:
- Alexa Fluor® secondary antibody OR streptavidin conjugates for superior photostability and brightness
- ProLong® Gold antifade reagent for reduced photobleaching
- Image-iT™ FX signal enhancer for an improved signal-to-noise ratio
A pack of two CultureWell® chambered coverglasses (C37005) is also included for convenient processing. Each Image-iT™ FX Kit provides sufficient materials to perform 50–100 assays.
Alexa Fluor® Secondary Detection Conjugates
The Alexa Fluor® dye conjugates are, without exception, the best conjugates we have ever tested. With excellent brightness and photostability, these fluorescent conjugates of streptavidin, goat anti–mouse, and goat anti–rabbit IgG are superior fluorescent secondary reagents for imaging.
- Learn More about Secondary Antibodies & Secondary Detection
Alexa Fluor® Dye Conjugates
|Labeling cells with the bright and photostable Alexa Fluor® dye conjugates yields stunning, high-quality images.|
ProLong® Gold Reagent - Ready-to-Use Antifade Reagent
Learn More about ProLong® Gold antifade reagent
ProLong® Gold Antifade Reagent
ProLong® Gold antifade reagent (top) provides significant photobleaching protection compared to PBS alone (bottom) over a 20-second time series. Images were acquired at 5-second intervals.
Image-iT™ FX Signal Enhancer - Eliminate Background Staining
Image-iT™ FX signal enhancer dramatically improves the signal-to-noise ratio of immunolabeled cells and tissues, allowing you to clearly visualize targets that would normally be indistinguishable due to background fluorescence. Background staining typically seen with fluorescent dyes is largely eliminated when Image-iT™ FX signal enhancer is applied to fixed and permeabilized cells before staining (Table 1).
- Learn More about Image-iT™ FX Signal Enhancer
Treatment with Image-iT™ FX signal enhancer
Treatment with Image-iT™ FX signal enhancer (top) largely eliminates nonspecific fluorescent dye binding in cells.
Image-iT™ FX signal enhancer
Treatment with Image-iT™ FX signal enhancer (bottom) largely eliminates nonspecific fluorescent dye binding in a tissue section.
Table 1 - Fluorescent Dyes Successfully Tested with the Image-iT™ FX Signal Enhancer*
|Alexa Fluor® 350
||Alexa Fluor® 633
|Alexa Fluor® 405
||Alexa Fluor® 635
|Alexa Fluor® 430
||Alexa Fluor® 647
|Alexa Fluor® 488
||Alexa Fluor® 660
||Oregon Green® 488
|Alexa Fluor® 532
||Alexa Fluor® 680
|Alexa Fluor® 546
||Alexa Fluor® 700
|Alexa Fluor® 555
||Alexa Fluor® 750
|Alexa Fluor® 568
|Alexa Fluor® 594
||Texas Red®, Texas Red-X
|Alexa Fluor® 610
* All dyes were conjugated to streptavidin and tested at 10 µg/mL.