Live-cell imaging can be challenging. Not only must staining be optimized for the particular assay readout, spectral compatibility and signal-to-noise level, but live-cell imaging of dynamic processes requires active observation over time.

The choice of medium is particularly important for time-lapse imaging and experiments where cells are exposed to ambient conditions for longer periods. Removing the labeling solution and rinsing with fresh medium will reduce the background for cells you want to continue culturing.

For imaging live cells as a final step (no further culture), a background suppressor can be used to optimize the signal.

Live-Cell Imaging Media

For reliable imaging results with live cells, it is essential that the cells be healthy and maintained as closely as possible to physiological temperature, pH, oxygen tension, and other conditions.  

With access to a range of live-cell imaging media and wash buffers you can optimize image clarity, signal-to-noise ratio, and cell viability throughout the imaging period.

  • PBS—ideal for cell washing and short-term imaging where prolonged incubation is not required
  • Live Cell Imaging Solution—an optically clear physiological solution that helps keep cells healthy for up to 4 hours
  • FluoroBrite™ DMEM—an optically clear solution for long-term imaging and subsequent cell culture

Oxidative stress detection in human osteosarcoma (U2-OS) cells.

Cell washing
Short-term imaging
Imaging up to 4 hrs
Long-term imaging
PBS, pH 7.4

Live Cell Imaging Solution

FluoroBrite™ DMEM

Background Suppression

BackDrop® Background Suppressor ReadyProbes™ Reagent is a set of novel reagents designed to effectively suppress background fluorescence in live-cell imaging samples. BackDrop® Background Suppressor is a direct-addition product supplied as a six-pack of ultra-convenient dropper bottles: two vials each of blue, green, and red background suppressors.

If you are observing high background signal or weak fluorescence in the blue, green, or red channels, see how BackDrop® Background Suppressor can improve your results by cutting the haze and increasing contrast.

BPAE cells in DMEM, 20% FBS without (–) and with (+) BackDrop® Background Suppressor.

Catalog # Name Size List Price (USD) Qty
R37603 BackDrop® Background Suppressor ReadyProbes® Reagent 1 kit 184.00