Loss of fluorescence through irreversible photobleaching processes can lead to a significant reduction in sensitivity, particularly when target molecules are of low abundance or when excitation light is of high intensity or long duration. The mechanisms associated with photobleaching are also implicated in phototoxicity, which may adversely impact cell viability and data quality.
To minimize photobleaching of experimental samples we have developed a series of antifade reagents, which have been shown to increase the photostability of many popular fluorophores in a variety of sample types. We offer choices for immediate imaging and for long-term storage and many of our formulations include a nuclear stain to achieve mounting and counterstaining in a single step.
ProLong® Gold antifade reagents
ProLong® Gold reagent significantly reduces fluorophore photobleaching across the visible spectrum, with little or no quenching of the fluorescence signal.
ProLong® Gold cures to a semi-rigid gel in a time period from two hours to overnight depending on sample thickness. For long-term storage, slides should be sealed.
- Learn more about ProLong® Gold antifade reagents
SlowFade® Gold antifade reagents
SlowFade® Gold antifade reagent was developed to provide the highest degree of antifade protection for the widest range of dyes.
Intended for short-term preservation (3–4 weeks), SlowFade® Gold reagent is a non-hardening mountant that does not cure over time. Samples may be viewed immediately after mounting.
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For added convenience and a reduction in the environmental impact associated with cold storage requirements, Life Technologies antifade reagents are provided in dropper bottles with storage right at your bench. In addition, these reagents are formulated with reduced sodium azide to help minimize environmental and health hazard exposure risks.