ProLong® Gold antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules for long-term storage and analysis. ProLong® Gold antifade reagents cure within 24 hours, forming a semi-rigid gel. Mounted samples can be saved for months with appropriate storage.
ProLong® Gold Antifade Reagent
- Inhibits photobleaching across the spectrum, even after prolonged storage
- Hard-setting formulation
- Available with or without DAPI
- Ready-to-use bench-top formulation
- Refractive index 1.46
ProLong® Gold Antifade Reagent is best for long-term analysis and storage. For immediate analysis and short-term storage choose one of our SlowFade® Gold antifade reagents.
ProLong® Gold antifade performance
ProLong® Gold antifade reagent outperforms all other commercially available antifade reagents because it preserves signal across the entire visible spectrum and causes little or no quenching of the initial fluorescent signal.
A 20-second time series showing the enhanced resistance to photobleaching afforded by ProLong® Gold antifade reagent.
Protection across the spectrum
ProLong® Gold antifade reagents offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.
ProLong® Gold Antifade Reagent outperforms competitors’ antifades across the visible spectrum.
Antifade reagents selection guide
|ProLong® Gold Antifade Reagent|
|Format||5 x 2 mL||5 x 2 mL|
ProLong® Gold performance across the spectrum
Resistance to photobleaching*
|Alexa Fluor® 350||346/442||65%|
|Alexa Fluor® 488||495/519||85%|
|Alexa Fluor® 546||556/575||67%|
|Alexa Fluor® 555||555/565||80%|
|Alexa Fluor® 568||578/603||93%|
|Alexa Fluor® 594||590/617||92%|
|Alexa Fluor® 647||652/668||100%|
|Alexa Fluor® 680||679/702||96%|
|*Expressed as a percentage of initial fluorescence intensity remaining following 30 seconds of continuous illumination using a 40x/1.4NA objective and a 100-watt Hg-arc lamp as the light source.|
For added convenience and a reduction in the environmental impact associated with cold storage requirements, Life Technologies antifade reagents are provided in dropper bottles with storage right at your bench. In addition, these reagents are formulated with reduced sodium azide to help minimize environmental and health hazard exposure risks.