Organelle stains

Organelle Selection

Organelles play a critical role in cellular function and the detection of specific organelles with either organelle specific antibodies or organelle selective stains and dyes are key in fluorescence imaging of cells and tissue. Organelle stains can be used as counterstains to help identify the location of specific proteins and targets of interest within the cell while detection of antibodies against proteins associated with specific organelles can lead to a better understanding of cellular function.

Find organelle specific antibodies and organelle stains using the selection guides below. Our organelle stains include organelle specific dyes and stains for live cell or fixed cell imaging as well as for flow cytometry. The organelle selection guide will help you find the right reagent for co-localization of cell structures.

 

Organelle—Adiposomes

Adiposomes are also known as lipid droplets and are prevalent in cells involved in lipid storage.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
LipidTOX™ Deep Red neutral lipid stain H34477 637/655
Developed for image-based high content screening (HCS) assays to characterize the toxic side effects of compounds on lipid metabolism in mammalian cell lines, and also for the monitoring of adipogenesis; highly specific for neutral lipids, supplied in ready-to-use formulations, require no wash steps
LipidTOX™ Green neutral lipid stain H34475 495/505
LipidTOX™ Red neutral lipid stain H34476 577/609
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Cytoplasm

The cytoplasm of the cell contains the functional organelles and is encompassed by the cell membrane. 

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
Calcein AM C3100MP 494/517
    Best single probe available for use in assays for cell adhesion, chemotaxis, and multidrug resistance; has better cell retention in viable cells than fluorescein, carboxyfluorescein, and BCECF; tends to have brighter fluorescence in a number of mammalian cell types
CellTrace™ calcein red-orange AM C34851 576/589
   
 
Intrinsically fluorescent; a useful cell tracer and indicator of cell viability; can be used in combination with blue and green-fluorescent probes
CellTrace™ Far Red DDAO-SE C34553 646/659 (pH-dependent spectra; values determined at pH 9)
   
 
A fixable, far red–fluorescent tracer for very long-term cell labeling; the succinimidyl ester (SE) reactive group forms a strong covalent attachment to primary amines that occur in proteins and other biomolecules on the inside and outside of cells
Organelle Lights™ Cyto-GFP O36227 485/520
 

(para-formaldehyde)
Cytoplasmic-residing due to a C-terminal nuclear export sequence
CellTracker™ Blue CMAC C2110 353/466
    Many cell types loaded with CellTracker™ probes remain fluorescent and viable 24 hours after loading, through cell division, and are not transferred to adjacent cells. An excellent choice for investigating cellular thiol levels, cell viability and cytotoxicity, transplantations, and cell fusion. Once inside the cell the CellTracker™ dye becomes impermeant and thus well retained with strong fluorescence and uniform cytoplasmic staining; simultaneous use of a CellTracker™ reagent and a calcium indicator may permit ratio measurements of intracellular calcium. CellTracker™ Blue CMAC has a low pKa and exhibits bright blue fluorescence in the cytoplasm at all physiological pH levels; viability in the presence of the reagent extends at least 72 hours
CellTracker™ Green CMFDA C7025 492/517
    CellTracker™ Green CMFDA has a relatively low pKa and exhibits bright green fluorescence in the cytoplasm at all physiological pH levels; CellTracker™ Green CMFDA is nonfluorescent until esterase cleavage occurs
CellTracker™ Orange CMTMR C2927 541/565
    CellTracker™ Orange CMTMR has a low pKa and exhibits bright orange fluorescence in the cytoplasm at all physiological pH levels; viability in the presence of the reagent extends at least 72 hours
CellTracker™ Red CMTPX C34552 577/602
    The longer-wavelength CellTracker™ Red CMTPX exhibits bright red fluorescence that is easily distinguished from that of blue-, green-, and far-red– fluorescent probes (including fluorescein-based CellTracker™ Green CMFDA); viability in the presence of the reagent extends at least 72 hours
Qtracker® 525 Cell Labeling Kit Q25041 525 ††
 
Qtracker® Cell Labeling Kits are best used with rapidly dividing cells or cells that are endocytically active, including common cell lines such as HeLa, CHO, U188, and 3T3
Qtracker® 565 Cell Labeling Kit Q25031 565 ††
 
Qtracker® 585 Cell Labeling Kit Q25001 585 ††
 
Qtracker® 605 Cell Labeling Kit Q25011 605 ††
 
Qtracker® 655 Cell Labeling Kit Q25021 655 ††
 
Qtracker® 705 Cell Labeling Kit Q25061 705 ††
 
Qtracker® 800 Cell Labeling Kit Q25071 800 ††
 
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Cytoskeleton

The cytoskeleton organelle plays a critical role in maintaining the structure of the cell and in intracellular transport of molecules and other organelles. The two main protein types of cytoskeleton are actin and tubulin.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
Alexa Fluor® 350 phalloidin A22281 346/442  
Alexa Fluor® dye–labeled phalloidins bind to F-actin in nanomolar concentrations and show equal affinity for large and small filaments; live-cell staining requires liposome delivery or microinjection of conjugates, compromised membranes, or unlabeled phalloidins; contrast between stained and unstained areas is extremely large; phalloidin-labeled actin remains functional
Alexa Fluor® 488 phalloidin A12379 495/519  
Alexa Fluor® 555 phalloidin A34055 555/565  
Alexa Fluor® 594 phalloidin A12381 581/609  
Alexa Fluor® 635 phalloidin A34054 633/647  
Alexa Fluor® 647 phalloidin A22287 650/668  
Alexa Fluor® 680 phalloidin A22286 679/702  
CellLight™ Actin-GFP C10582 488/510
 
Fusions of fluorescent proteins to the N-terminus of β-actin in mammalian cells; allow visualization of cytoskeletal components in live cells or cytoskeletal rearrangements in HCS cytotoxicity assays
Cellular Lights™ Actin-RFP C10127 555/584
 
CellLight™ Tubulin-GFP C10613 488/510
 
Fusions of fluorescent proteins to the N-terminus of β-tubulin that allow live-cell imaging of microtubules; to monitor tubulin changes as an indicator of cytotoxicity; also for following cell division in transiently transduced cells, including primary and stem cells
CellLight™ Tubulin-RFP C10614 555/584
 
CellLight™ MAP4-GFP C10598 488/510
 
Fusions of fluorescent proteins to microtubule associated protein 4 (MAP4), enabling visualization of microtubules in live cells that can also readily be fixed for end-point studies such as HCS-based cytotoxicity analyses
CellLight™ MAP4-RFP C10599 555/584
 
Rhodamine phalloidin R415 540/565
  Conjugated to a popular orange-fluorescent dye, tetramethylrhodamine (TRITC); convenient probe for labeling, identifying, and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures, or cell-free experiments
TubulinTracker™ Green T34075 494/522
    Green-fluorescent staining of polymerized tubulin in live cells
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Endoplasmic reticulum

The endoplasmic reticulum organelle comprises a network of tubules, vesicles and cisternae that intertwine throughout the cytoplasm. Rough ER synthesizes proteins while smooth ER is responsible for synthesis of carbohydrates and steroids. Sarcoplasmic reticulum is involved in calcium regulation.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
Concanavalin A, Alexa Fluor® 594 conjugate C11253 590/617    
  Brighter fluorescence than our Texas Red® dye conjugate of concanavalin A, and more photostable; concanavalin A selectively binds to α-mannopyranosyl and α-glucopyranosyl residues
ER-Tracker™ Green E34251 504/511
 
ER Tracker™ Green and Red are live-cell permeant and highly endoplasmic reticulum-selective BODIPY®-based dyes; when cells are stained using the provided protocol, staining patterns are partially retained after aldehyde fixation
ER-Tracker™ Red E34250 587/615
 
CellLight™ ER-GFP C10590 485/510
 

(paraformaldehyde)
Targeted specifically to nucleus via the endoplasmic reticulum organelle signal sequence of calreticulin and KDEL (ER retention signal)
CellLight™ ER-RFP C10591 555/584
 

(paraformaldehyde)
SelectFX® Alexa Fluor® 488 ER Labeling Kit S34200 495/519  
  Kit for endoplasmic reticulum organelle labeling in fixed cells, providing a primary antibody directed against ER-associated disulfide isomerase (PDI), an Alexa Fluor® 488 dye-labeled secondary antibody, PBS, blocking solution, fixative and permeabilization solutions
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Golgi complex

The Golgi complex is the organelle where macromolecules, such as proteins, lipids and carbohydrates, are prepared for either secretion from the cell or for use by other organelles within the cell.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
Anti–human golgin-97 antibody (detection with an Alexa Fluor® dye–labeled secondary antibody) A21270 NA  
  Detects the peripheral membrane protein golgin-97 on cytoplasmic face of the Golgi complex; cross-reacts with the golgin-97 protein from most species.
BODIPY® FL C5-ceramide complexed to BSA D3521, B22650 505/511
 
Selective staining of the Golgi complex with applications for lipid metabolism and trafficking studies; brighter, more photostable than NBD conjugates; concentration-dependent shift from green to red in the Golgi complex; good for outlining cell boundaries for observation of morphogenetic movements and for measuring rates of lipid synthesis by Schwann cells
BODIPY® TR ceramide complexed to BSA D7540, B34400 589/617
 
Selective staining of the Golgi complex with applications for lipid trafficking studies, brighter and more photostable than the NBD conjugates; displays no concentration-dependent emission
Lectin HPA from Helix pomatia (edible snail), Alexa Fluor® 488 conjugate L11271 495/519       Lectins selectively bind to terminal α-N-acetylgalactosaminyl residues—an intermediate sugar added in O-linkage to serine and threonine residues in cis-Golgi cisternae and then substituted with galactose and sialic acid in the trans-Golgi organelle; for each cell type or tissue it is necessary to empirically determine appropriate lectins and staining conditions
Lectin HPA from Helix pomatia (edible snail), Alexa Fluor® 647 conjugate L32454 650/668      
NBD C6-ceramide N1154 466/536
 
 
 
Classic structural marker for the Golgi complex
CellLight™ Golgi-GFP C10592 488/510
 

(para-formaldehyde)
Fusion proteins targeted specifically to the Golgi complex via the human Golgi-resident enzyme N-acetylgalactosaminyltransferase- 2
CellLight™ Golgi-RFP C10593 555/584
 

(para-formaldehyde)
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Intracellular membranes

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
CellTrace™ BODIPY® TR C34556 598/625
Accumulates in cellular membranes including plasma membrane and organelle membranes (i.e., mitochondria, ER, and Golgi apparatus); an excellent counterstain for cells and tissues expressing GFP
Image-IT® LIVE Intracellular Membrane and Nuclear Labeling Kit I34407       350/461 (Hoechst 33342), 598/625 (BODIPY® dye)
Contains CellTrace™ BODIPY® TR methyl ester and Hoechst 33343 for selective staining of the intracellular membranes and nuclei of live, GFP-transfected cells
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Lysosomes

Lysosomes are acidic organelles that are responsible for digesting and degrading damaged organelles and other cellular debris. They also play an important role in autophagy.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
Image-iT® LIVE Lysosomal and Nuclear Labeling Kit I34202       350/461 (Hoechst 33342), 577/590 (LysoTracker® Red DND-99)
 
Contains LysoTracker® Red DND-99 dye and Hoechst 33342 for highly selective staining of lysosomes and nucleus in live GFP-transfected cells
LysoTracker® Blue DND-22 L7525 373/422
 
 
Highly selective for acidic organelles at nanomolar concentrations, shows minimal staining of mitochondria, for labeling and tracking of acidic organelles in live cells, loads rapidly
LysoTracker® Green DND-26 L7526 504/511
 
 
Taken up very rapidly by live cells; imaging using this dye is best between 1 and 5 minutes after dye is added
LysoTracker® Red DND-99 L7528 577/590
 
Rapid loading, highly selective for acidic organelles at nanomolar concentrations, shows minimal staining of mitochondria, for labeling and tracking of acidic organelles in live cells
LysoSensor™ Blue DND-167 L7533 373/425
 
 
LysoSensor™ dyes exhibit a pH-dependent increase in fluorescence upon acidification (more specific than traditional organic stains); they come in multiple colors with different pKas and are used singly (or potentially in combination) to investigate the acidification of lysosomes and alterations of lysosomal function or trafficking that occur in cells Nonfluorescent at pH 7; increased fluorescence in acidic range
LysoSensor™ Green DND-153 L7534 442/505
 
 
Nonfluorescent at pH 7; increased fluorescence in acidic range
LysoSensor™ Yellow/Blue dextran, 10,000 MW L22460       335, 381/452, 521
 
 
Fluorescence changes from blue (near neutral pH) to yellow (in acidic compartments); not cell permeant (must be microinjected or loaded by endocytosis); compatible with microscopy and flow cytometry
LysoSensor™ Yellow/Blue DND-160 L7545       329, 384/440, 540
 
 
pH-dependent dual excitation and dual emission peaks for ratiometric imaging; yellow fluorescence in acidic organelles, blue fluorescence in less acidic organelles
CellLight® Lysosomes-GFP C10596 488/510
 

(para-formaldehyde)
Fusion proteins targeted to lysosomal associated membrane protein 1; for live-cell visualization of lysosomes and any process in which they are involved—phagocytosis, endocytosis, autophagy
CellLight® Lysosomes-RFP C10597 555/584
 

(para-formaldehyde)
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Membrane Trafficking

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
CellLight™ Early Endosomes-GFP C10586 488/510
 

(paraformaldehyde)
Fusion protein targeted to Rab5a, an early endosomal marker; allows following live-cell endosomal movement; cells can also be fixed for end-point studies
CellLight™ Early Endosomes-RFP C10587 555/584
 

(paraformaldehyde)
Organelle Lights™ Synaptophysin-GFP C10080 488/510
 

(paraformaldehyde)
Fusions of fluorescent proteins to synaptophysin; for live-cell studies of endocytosis, exocytosis, synaptic vesicles, and membrane–synaptic vesicle complexes
CellLight™ Synaptophysin-RFP C10610 555/584
 

(paraformaldehyde)
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Learn more about CellLight™ Ready-To-Use Fluorescent Protein Based Reagents

Organelle—Mitochondria

Mitochondria are small organelles that house the energy producing machinery of the cell.  They are involved in many key cellular processes including cell death, cell cycle, cell growth as well as energy production.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
Anti-OxPhos Complex V d subunit antibody (detection with an Alexa Fluor® dye–labeled secondary antibody) 459000    
  One of the many important mitochondria specific antibodies for investigating mitochondrial biogenesis and mitochondrion-related diseases by western blot, native gel electrophoresis, immunohistochemistry, and other techniques;
Image-iT® LIVE Mitochondrial and Nuclear Labeling Kit I34154       350/461 (Hoechst 33342), 579/599 (MitoTracker® Red)
 
Contains MitoTracker® Red CMXRos dye and Hoechst 33342 for highly selective mitochondrial and nuclear staining in live GFP-transfected cells
JC-1 T3168      514/529 (monomer form), 485–589/590 (J-aggregate form)
   
 
Widely used cationic dye that is dependent upon mitochondrial membrane potential for accumulation; JC-1 monomer (low concentration) is green, whereas accumulation at higher concentration shifts emission to the red, allowing ratiometric membrane potential measurements
MitoTracker® Green FM M7514 490/516
 
 
Staining does not appear driven by mitochondrial function. Useful counterstain for mitochondrial mass. Has been reported as showing specific staining after fixation in certain cells.
MitoTracker® Red FM M22425 581/644
    Good live-cell stains, and well suited for multicolor labeling experiments; all are well retained after fixation except MitoTracker® Red FM
MitoTracker® Orange CMTMRos M7510 554/576
 
MitoTracker® Red CMXRos M7512 579/599
 
MitoTracker® Deep Red FM M22426 644/665
 
MitoTracker® Orange CM-H2TMRos M7511 554/576
 
Does not fluoresce until it enters live cells where it is oxidized to the fluorescent mitochondrion-selective probe MitoTracker® Red CM-H and is sequestered in mitochondria
MitoTracker® Red CM-H2XRos M7513 579/599
 
CellLight® Mitochondria-GFP C10600 485/510
 
(paraformaldehyde)
Targeted specifically to mitochondria via the leader sequence of E1 pyruvate dehydrogenase
CellLight® Mitochondria-RFP C10601 555/584
 
(paraformaldehyde)
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Nuclear envelope

The nuclear envelope acts as a barrier, separating the cytoplasm from the nucleus. It consists of a lipid bilayer that contains nuclear pores and key nuclear membrane proteins.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
Organelle Lights™
 NE-GFP
O36213 485/520  

(paraformaldehyde)
Targeted specifically to nucleus via the nesprin-1α C-terminal transmembrane domain
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Nucleoli

The nucleoli of the cell are non-membrane bound bodies found within the nucleus of the cell and are the site where ribosomal RNA is transcribed.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
SYTO® 81
Orange nucleic acid stain
S11362 530/544   Nucleolar
SYTO® RNASelect™ Green S32703 490/530
(bound
to RNA)
  RNA selective, exhibiting increased fluorescence with RNA binding, prominent nucleolar staining, weak mitochondrial staining
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Nucleus

The nucleus of the cell is responsible for regulation of gene expression and is a membrane-bound organelle that comprises the nuclear envelope, nucleoli, and nuclear lamina.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
7-AAD A1310 546/647  
  Nuclear stain, GC selective, weakly permeant in some cell types
CellLight™ Histone 2B-GFP C10594 488/510
 
Alternatives to DNA staining for localizing the nucleus and chromosomes; allow localization of histones and fol lowing of cell division in live cells
CellLight™ Histone 2B-RFP C10595 555/584
 
DAPI D1306 358/461  
  Excellent nuclear counterstain, useful for mycoplasma detection, semipermeant at high concentration
Ethidium homodimer 1 E1169 528/617  
  One of the highest-affinity fluorescent probes available for nucleic acid staining; binds to dsDNA, ssDNA, RNA, and oligonucleotides with a significant fluorescence enhancement (>40-fold); dye intercalation is not sequence selective
Hoechst 33342 H1399, H3570 350/461
  Nuclear counterstain, water soluble, relatively nontoxic to cells, slightly more permeant than Hoechst 33258
Organelle Lights™ Nuc-CFP O36218 435/485
 

(paraformaldehyde)
Fluorescent proteins targeted specifically to nucleus via the SV40 nuclear localization sequence; allows visualization of the nucleus without utilizing DNA-binding dyes; can also be used as a segmentation marker in end-point HCS studies
Organelle Lights™ Nuc-GFP O36209 485/520
 

(paraformaldehyde)
CellLight™ Nucleus-GFP C10602
488/510
 

(paraformaldehyde)
CellLight™ Nucleus-RFP C10603 555/584
 

(paraformaldehyde)
Propidium iodide P3566 530/625  
  One of the most commonly used fixed-cell nuclear stains
SelectFX® Nuclear Labeling Kit, for fixed cells (DAPI, SYTOX® Green, 7-AAD, TO-PRO®-3 iodide) S33025 Several  
  A sampler kit of our most popular nuclear stains with optimized protocols for product use
SYTO® 9 green fluorescent nucleic acid stain S34854 483/503
 
Live-cell SYTO® dyes are permeant to eukaryotic and prokaryotic membranes, exhibit affinity for DNA and RNA, and display enhanced fluorescence upon binding Live-cell nuclear counterstain, useful for bacterial viability
SYTO® 14 green fluorescent nucleic acid stain S7576 517/549 (DNA),
521/547 (RNA)
 
Live-cell nuclear counterstain, twice as fluorescent when complexed with RNA as with DNA
SYTO® 82 orange fluorescent nucleic acid stain S11363 541/560
 
Live-cell nuclear counterstain
SYTO® 59 red fluorescent nucleic acid stain S11341 622/645
 
Live-cell nuclear counterstain
SYTO® 61 red fluorescent nucleic acid stain S11343 628/645
 
Live-cell nuclear counterstain, live and dead bacterial stain
SYTOX® Green nucleic acid stain S7020 504/523  
  Highest-affinity SYTOX® stain, fixed-cell stain, useful as a dead-cell stain, 500× increase in fluorescence upon nucleic acid binding, can be used in conjunction with blue and red stains for multiplexing
SYTOX® Orange nucleic acid stain S11368 547/570  
  Fixed-cell nuclear stain, some mitochondrial staining, shorter-wavelength emission than propidium iodide, useful as a dead-cell stain, 500× increase in fluorescence upon nucleic acid binding, can be used in conjunction with blue and green stains for multiplexing
TO-PRO®-3 iodide T3605 642/661  
  Ultrasensitive dsDNA detection, dead-cell stain, electrophoresis prestain, used as an alternative to annexin conjugates for analyzing apoptotic cells
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Peroxisomes

Peroxisomes are membrane bound organelles that reside in the cytoplasm of the cell. They are responsible for removing harmful peroxides from the cell as well as being involved in fatty acid metabolism.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
CellLight™ Peroxisome-GFP C10604 488/510
 

(paraformaldehyde)
Targeted specifically to peroxisome, via the peroxisomal C-terminal targeting sequence
SelectFX® Alexa Fluor® 488 Peroxisome Labeling Kit S34201 495/519  
  Kit for peroxisome labeling in fixed cells, providing a primary antibody directed against peroxisomal membrane protein 70 (PMP 70), an Alexa Fluor® 488–labeled secondary antibody, PBS, blocking solution, fixative, and permeabilization solutions
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.

Organelle—Plasma membrane

The plasma membrane separates the interior of the cell from the extracellular components. It consists of mainly proteins and lipids and is instrumental in the signaling pathways as well as ionic homeostasis.

Product Cat. no.
Emission Color*
Ex/Em† Live‡ Fixed§ Fixable** Key attributes
Alexa Fluor® 350 WGA W11263 346/442
 
Alexa Fluor® labeled wheat germ agglutinins are versatile cationic probes for detecting glycoconjugates, i.e., N-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues on cell membranes; useful in fixed and live cells that have not been permeabilized; best if fixed rapidly after staining
Alexa Fluor® 488 WGA W11261 495/519
Alexa Fluor® 555 WGA W32464 555/565
Alexa Fluor® 594 WGA W11262 590/617
Alexa Fluor® 633 WGA W21404 632/647
Alexa Fluor® 647 WGA W32466 650/665
Alexa Fluor® 680 WGA W32465 679/702
CellTracker™ CM-DiI C7001 553/570
 
Bright, photostable, with excellent cellular retention; particularly suitable for cell tracking and long-term labeling of intracellular membranes, liposomes, viruses, and lipoproteins; aldehyde fixable
FM® 1-43FX F35355 510/626
 
FM®1-43 FX and FM®4-64FX exhibit substantially enhanced fluorescence upon insertion into the membrane and can be used for plasma membrane labeling when fixative is applied quickly after cell labeling. They are used to label the plasma membrane and to study vesiculation; they are easily applied to cells, nontoxic, water soluble, aldehyde fixable, and nonfluorescent in aqueous environments
FM® 4-64FX F34653 565/744
 
Image-iT® LIVE Plasma Membrane and Nuclear Labeling Kit I34406       350/461 (Hoechst 33342), 590/617 (Alexa Fluor® 594 WGA)
Contains Alexa Fluor® 594 WGA and Hoechst 33343 for selective staining of the plasma membrane and nucleus of live GFP-transfected cells
Lipophilic Tracer Sampler Kit (DiI, DiO, DiR, DiD, DiA) L7781 Several
 
An assortment of the lipophilic carbocyanine dyes commonly used for membrane staining
CellLight™ Plasma Membrane-CFP C10606 440/480
 

(paraformaldehyde)
Fluorescent proteins targeted to the plasma membrane through the myristoylation/palmitoylation sequence from Lck tyrosine kinase; bright, specific plasma membrane staining
CellLight™ Plasma Membrane-GFP C10607 488/510
 

(paraformaldehyde)
CellLight™ Plasma Membrane-RFP C10608 555/584
 

(paraformaldehyde)
* Gray represents fluorescence emission that is beyond the limit of human vision. † Ex/Em = Fluorescence excitation and emission maxima in nm. ‡ Stains live cells. § Stains fixed cells. ** Staining pattern is fixable. NA = Not applicable.