High-content analysis (HCA) or high-content screening (HCS) describes a set of automated analytical methods using integrated microscopy, image processing, and visualization tools to extract data from cells or cell populations and report quantitative results. HCS typically employs fluorescence imaging of samples in a high-throughput format, and reports on quantitative measurements such as spatial distribution of targets in cells and individual cell and organelle morphology. In particular, HCS combines multiple measurements per cell and is applied to multiple cell populations in the course of an experiment.

Thermo Scientific™ high-content instruments range from the modular and flexible Thermo Scientific™ ArrayScan™ XTI and XTI Infinity Configuration High Content Analysis (HCA) Readers to our affordable, benchtop Thermo Scientific™ CellInsight™ CX5 High Content Screening (HCS) Platform. All platforms feature state-of-the-art hardware and are designed for ease of use and maintenance.

More information on individual systems

photo of the ArrayScan™ XTI Infinity Configuration

Reagents for high-content screening

Drawing on decades of experience in fluorescence imaging, Molecular Probes® HCS products were developed with special considerations for automated imaging:

  • Cell and nuclear masks for automated demarcation
  • Robust functional probes for cell health interrogation
  • Flexible assay workflow for automated processing
  • Broad fluorophore choice for easy multiplexing
  • Validated on multiple imaging platforms

Download the HCS Selection Guide


HCS segmentation tools and demarcation

Clear identification of cell and nuclear boundaries in automated imaging requires fiducial markers that will label cellular structures robustly and reproducibly. Cell masks are designed specifically for automated imaging. Cell structure labels are widely used in microscope imaging and high-content analysis.


HCS cell health and cytotoxicity assays

We offer a diverse selection of imaging and microscopy assays for the analysis of cell viability, proliferation apoptosis, cell cycle and DNA repair, migration, adhesion and other cellular functions. Many of these assays have been validated specifically for HCS platforms.