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  • BacMam–based Organelle Lights™ and Cellular Lights™ Fluorescent Protein expression products
  • TC-FlAsH™ TC-ReAsH™ labeling products for tetracysteine tags
  • Fluorescent protein expression vectors

BacMam–based Organelle Lights™ and Cellular Lights™ Fluorescent Protein expression products

Organelle Lights™ and Cellular Lights™ fluorescent protein expression reagents are prepackaged and ready-to-use fluorescent protein fusion constructs for specific targeting to subcellular organelles and structures. These reagents are available in a variety of colors for convenient multiplexing and colocalization studies, and their targets include the cytoskeleton, mitchondria,  nucleus, plasma membranes, ER, the Golgi apparatus, and peroxisomes.

Organelle Lights™ Fluorescent Protein Expression Reagents


Available Fluorescent Protein Colors


(Excitation/Emission)

Organelle Lights™ CFPGFPYFPOFPRFP
(440/480)(488/510)(514/528)(548/565)(555/584)
Cytoplasm
O36227


Endoplasmic Reticulum
O36212
O36223
Endosomes
O10104


Golgi
O36215
O36224O10098
Lysosomes



O10100
Mitochondria
O36210
O36222
Nuclear Envelope
O36213


NucleusO36218O36209  O10099
Peroxisome
O36211
 
Plasma MembraneO36216O36214 O36226
Synaptophysin
C10080


Null virus (control)

C10130 

Learn more about Organelle Lights™ reagents.

Cellular Lights™ Fluorescent Protein Expression Reagents


Available Fluorescent Protein Colors


(Excitation/Emission)

Cellular  Lights™ CFPGFPYFPOFPRFP
(440/480)(488/510)(514/528)(548/565)(555/584)
Actin
C10126


C10127
CSF1r
C10078



EXOC1

C10079


Histone 2B

C10128


C10129
MAP4
C10105



Tubulin
C10106


C10112
Null virus (control)

C10130 

Learn more about Cellular Lights™ reagents.

TC-FlAsH™ TC-ReAsH™ labeling products for tetracysteine tags

The TC-FlAsH™ TC-ReAsH™ labeling products are based on the FlAsH and ReAsH tetracysteine tag technology developed by Tsien and colleagues. The technology consists of two nonfluorescent biarsenical compounds that become highly fluorescent upon binding to a specific sequence tetracysteine motif. The best protein sequence for biarsenical compound binding is CCPGCC. The TC-FlAsH™ TC-ReAsH™ labeling products provide researchers with a unique set of tools for studying cellular processes and pathways with the following features:

  • Quick tagging—proteins can be visualized as soon as translation occurs
  • Convenience—tetracysteine tag can be easily subcloned onto the protein expression sequence of interest
  • Reliability—small tetracysteine tag does not interfere with the behavior endogenous proteins
  • Multiplexing flexibility—choice of green or red labeling on tetracysteine tagged protein
  • Low background—new wash buffer greatly reduces nonspecific staining

FlAsH-EDT2 labeling reagent
ReAsH-EDT2 labeling reagent
Choice of fluorescent colors using the TC-FlAsH™ TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit. An actin–TC-Tag fusion protein was used in a transient transfection experiment of CHO cells. Separate samples of transfected cells were stained with FlAsH-EDT2 labeling reagent (left) and ReAsH-EDT2 labeling reagent (right). Actin staining is very strong in both samples; only a low level of background fluorescence is seen in the nontransfected neighboring cells. The experiment shows that a single tagged protein can be detected with either a green- or red-fluorescent label.

Ordering Information

Fluorescent protein expression vectors

The Vivid Colors™ Fluorescent Protein Vectors are Life Technologies' next-generation cloning and expression tools that feature Aequorea victoria–derived fluorescent proteins for simple, noninvasive detection of recombinant proteins. With Gateway® and TOPO® cloning vector options, cloning, expression, and viewing have never been so easy!

  • Be confident using well-published and trusted fluorescent proteins derived from Aequorea victoria jellyfish
  • See fluorescent reporter proteins in 24 hr
  • Use multiple colors for co-visualization applications
  • Easily clone into Life Technologies' powerful Gateway® and TOPO® vectors


Learn more about Fluorescent Protein Vectors