TC FlAsH: Expression Analysis Detection Kits
- Rapid, direct labeling of purified or crude cell lysate means no gel staining needed
- Visualize your protein of interest in gel and even within the cassette alongside reference Benchmark ™ protein ladder as well as total protein in contrasting color
- Westerns are no longer necessary to confirm TC-tagged proteins
The contrasting stain in either red or orange allows visualization of your total protein alongside the Benchmark™ total protein ladder for reference.
- The small 6-amino acid 1 kD tetracysteine tag (CCPGCC) and FlAsH stain described by Roger Tsien offer the high specificity, high sensitivity, and flexibility needed for protein labeling.
- Molecular Probes® now offers tools for rapidly identifying TC-tagged proteins in gels as well as in-cell for microscopy.
- Imaging will require a Safe Imager, UV box, or laser scanner and appropriate filters.
Table 1 - The specificity of the TC-Tag provides accuracy as well as sensitivity
|TC-FlAsH vs. Other Tags||TC-FlAsH||Covalys SNAP tag||LUMIO||Invision His-tag|
|Sensitivity in cassette||at 488: 4 ng
at UV: 60 ng
|Sensitivity out of cassette||at 488: 250 pg
at UV: 1 ng
|at 488: < 20 pg||at UV: 30 ng||at UV: 5 ng|
|Total protein sensitivity at either 665 or 620 nm||Orange: IN 100 ng/OUT 12 ng
Red: IN 100 ng/ OUT 50 ng
|Detection||Excitation peak (nm)||Emission peak (nm)||Filter recommendation|
|TC-tagged protein||500 mid-UV||535 mid-UV||520LP
|Orange total protein||580 mid-UV||620 mid-UV||580LP
|Red total protein||650 mid-UV||665 mid-UV||675LP|