Find answers to frequently asked questions about the Tali® Image Cytometer such as basic technical aspects, physical features, computer compatibility and archiving, and cellular analysis capabilities.
How long does it take to run an assay on the Tali® Image Cytometer?
|Assay Time (sec)|
|Number of fields||Quick Count||Viability||Apoptosis||GFP||GFP + Viability||RFP||RFP + viability||GFP + RFP|
As we test more cell types, data and information will be available on the website.
|Product||Green Channel||Red Channel||For use with:||Cat. No.|
|Tali® Viability Kit—Dead Cell Green||SYTOX® Blue dye||N/A||RFP cells||T10787|
|Tali® Viability Kit—Dead Cell Red||N/A||Propidium iodide (PI)||GFP cells||T10786|
|Tali® Apoptosis Kit||Annexin V—Alexa Fluor® 488 dye||Propidium iodide (PI)||Apoptosis studies||T10788|
*Note: Assays optimized for the Tali® cytometer include reagents selected specifically to minimize spectral overlap between channels. The fluorescence profiles of other dyes may be compatible with the Tali® cytometer, however they have not been fully tested and validated for use with this platform.
|Channel||Excitation||Emission||Use or Fluorophores Detected|
|Bright field||White LED||--||Visualize and count cells|
|Green||466/40 nm (Blue LED)||525/50 nm||Green fluorescent protein (GFP), Alexa Fluor® 488 dye, SYTOX® Blue dye|
|Red||543/22 nm (Green LED)||585 LP||Red fluorescent protein (RFP), Propidium iodide (PI)|
- Green channel: Emerald, EGFP
- Red channel: DsRed, tagRFP
The following fluorescent proteins have the appropriate spectral properties for the Tali® cytometer, but have not been tested yet:
- Red channel: mOrange, mKO, cdTomato
What are the physical dimensions of the Tali® cytometer?
- 11.5 in (H) X 11.5 in (W) X 17.5 in (D)
- 29.2 cm (H) X 29.2 cm (W) X 44.5 cm (D)
- Weight: 19.4 lbs, or 8.8 kg
What slides are compatible with Tali® Image Cytometer?
Does Tali® Image Cytometer require a computer?
What cell types have been tested?
For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.