Imunnofluorescence imaging

For critical research, you need reliable protein detection, and Life Technologies provides a complete suite of labeling tools for any level of target abundance. Our fluorescent products portfolio is the most peer-referenced in life science research.

Check out this simple guide and see which tool is right for your protein.

  • High-abundance target—primary conjugate, no amplification needed
  • Medium- to high-abundance target—secondary conjugate, modest amplification
  • Medium- to low-abundance target—streptavidin conjugate, elevate the signal
  • Low-abundance target—enzyme amplification, complex process for maximum signal enhancement

Immunofluorescence selection guide

 

High-abundance target

e.g., tubulin

Medium-abundance target

e.g., golgi or mitochondria

Low-abundance target

e.g., receptors or cell junction

 

Labeled primary

 

Secondary
detection

 

Streptavidin

 

TSA™
technology

Optimum signal for brightness/sensitivitySignal too strongSignal too weak

Primary Antibodies

Primary antibodies   Going direct with your choice of label has never been easier.
Life Technologies offers a wide variety of primary antibodies against key signaling proteins, cellular markers, and organelles, as well as cell junctions and CD markers. Primary antibodies provide direct detection for high-abundance targets, and high target specificity when using amplified detection methods.

Secondary Antibodies

Secondary antibodies   Secondary antibodies with a choice of dyes provide that extra signal and assay flexibility.
Life Technologies offers access to some of the most cited secondary antibodies in life science research, including Alexa Fluor® dye–labeled probes and Qdot® conjugates, as well as classic dyes in a wide range of colors.

Streptavidin

Streptavidin   Streptavidin conjugates with a choice of labels boost your signal from low-abundance targets.
For improved detection sensitivity, streptavidin-based amplification techniques are widely used in fluorescence imaging to detect primary and secondary antibodies, ligands, and toxins, or DNA probes for in situ hybridization.

TSA™ Technology

TSA™ technology   Simple amplification protocols with a choice of wavelengths for rare targets.
With TSA™ technology, you can see and measure low-abundance targets that are not detectable by conventional means. Tyramide-based signal amplification provides superior sensitivity without compromising resolution.

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