Small Animal In Vivo Imaging

Small animal in vivo imaging is an exciting new frontier in biology.

Novel techniques for imaging biological processes in live animals is changing how cellular activity, effects of drugs, and disease progression are monitored. We offer a range of reagents and kits with Molecular Probes® near-infrared (NIR) fluorescent dyes and conjugates along with our Qdot® nanocrystals. Areas of focus include Vascular Imaging and Inflammation as well as optimal Antibody Labeling.

Antibody Labeling
SAIVI™ Antibody Labeling

Non-invasive optical imaging in small animals is best achieved with antibodies or proteins labeled with near-infrared (NIR) fluorophores.

Learn More about SAIVI™ Antibody Labeling

Qdot® Nanocrystals In Vivo
Qdot® Nanocrystals for In vivo Applications

The unique photochemical properties of Qdot® nanocrystals offer an exciting and powerful tool for opening new directions in in vivo imaging applications.

Learn More about Qdot® Nanocrystals for In Vivo Applications

Vascular Imaging
Vascular Imaging

Optical-based in vivo imaging of vasculature is an emerging modality for studying vascular structure, function and angiogenesis.

Learn More about Vascular Imaging and Small animal In Vivo Imaging

Inflammation
Inflammation

Tracking recruitment to and imaging at the site of inflammation will be important in vivo imaging processes to assess inflammatory diseases and to further study the link between chronic inflammation and cancer.

Learn More about Inflammation and Small animal In Vivo Imaging

Reagents For Potential SAIVI™ Applications
We have quite a few reagents and dyes that could potentially be used for small animal in vivo imaging. Although not validated in this application, they do meet  the chemical and photochemical criteria needed.

  • Check Out Other SAIVI™ Conjugates and Dyes
Technical Resources for Small Animal In Vivo Imaging
SAIVI™ Reagents and Kits used in Scientific Posters & Presentations

Optical Imaging Probes for in vivo Tumor Biology. T.H. Steinberg, J.K. Nyhus, B. Aggeler, L. Greenfield, and L. Leong
Cambridge Healthtech Institute's Third Annual IN VIVO MOLECULAR IMAGING: Moving from Discovery to Pre-Clinical to Clinical Applications, Nov 2006

We have developed contrast agents with transient vascularimaging properties ranging from a few minutes to a few hours. We show the determination of an optimal degree of labeling (DOL; mole fluorophore/mole probe) for targeted antibodies with an NIR Alexa Fluor® dye and demonstrate transient imaging and multiplexed imaging of vascuarlized tumors in nu/nu mice.

Products Used:  Alexa Fluor® 750 SAIVI™ Antibody Labeling Kit, Alexa Fluor® 680 SAIVI™ Antibody Labeling Kit, QTracker® 655 Non-Targeted Quantum Dots, &  SAIVI™ Alexa Fluor ®750 Injectable Contrast Agent, bovine serum

Check out this poster as well:
Vascular Contrast Agents for Optical in vivo Imaging. J.K. Nyhus, T.H. Steinberg, L. Leong and L. Greenfield. SMI, 2006.

Frequently Asked Questions about Small Animal In Vivo Imaging

    Q.   What is DOL?

    A.  Degree of Labeling (DOL) describes the number of fluorophores per antibody.   For in vivo labeling experiments, the DOL is restricted to a narrow range because it has significant consequences for the biodistribution and clearance of the probe.  We have determined that the DOL range for the NIR Alexa Fluor® dyes is 1.5 to 3 molecules per antibody for optimal optical in vivo imaging.

View More FAQs for SAIVI™
SAIVI™ in Action

vascular labeling in a human tumor xenograft in a living mouse with the SAIVI™ Alexa Fluor® 750 injectable Contrast Agent
View a short video that demonstrates the vascular labeling and capillary leakage in a human tumor xenograft in a living mouse with the SAIVI™ Alexa Fluor® 750 injectable Contrast Agent *bovine serum albumin* as imaged using the CRi Maestro™ imaging system.