Antibodies for Flow Cytometry

Life Technologies™ has a diverse array of highly specific RUO, ASR, and IVD primary antibodies to targets for clinical and research settings.  These antibodies are validated for flow cytometry and include CD markers, antibodies for cancer, Immunology, stem cell studies, cell signaling, cytokines, and chemokines, as well as for key targets involved in cellular processes, plus much more. Find new antibodies for flow cytometry.

ASR antibodies for flow cytometry

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About Antibodies for Flow Cytometry

Range of Antibody conjugates Available
We offer 24 different fluorescent dyes (including the Alexa Fluor® family of dyes, and Pacific Blue™ and Pacific Orange™ dyes), proteins (PE, PerCP, and APC), and Qdot® nanocrystals conjugated to a wide range of antibodies to further expand your multiparametric flow cytometry capabilities.  This range of dyes is compatible with all flow cytometers.  Download our instrument selection guide to see which dyes are compatible with your instrument.


Wide Range of Colors at Work
The availability of more color options allows researchers to be more efficient in their experiments by obtaining more information with less sample, in less time. Through multiplexing, experimental error is diminished by minimizing the number of samples used to obtain results. Our wide selection of fluorophores allows researchers to select panels with minimal spectral overlap (Figure 1).

 

Wide selection of fluorophores

 

(click to enlarge)

Figure 1. Eight-color immunostain combination with low compensation values. Human peripheral blood leukocytes (PBLs) were stained with Qdot® 605–anti-CD4, Qdot® 655–anti-CD3, Qdot® 705–anti-CD45, FITC–anti-CD2, R-PE–anti-CD16+CD56, R-PE–Cy®7-anti-CD19, APC–anti-CD14, APC–Alexa Fluor® 750–anti-CD8. Samples were run on an LSR II flow cytometer. Plots are gated on lymphocytes by side scatter/CD45. Axes are labeled with the filters used; plots are labeled with compensation values.

Qdot® nanocrystals offer flexibility—excited by 405 nm or 488 nm, maximizing violet laser use; compatibility—combine with existing organic dyes, increase the number of detectable parameters; stability—does not degrade over time like tandem conjugates, for greater reproducibility; and minimal single laser compensation—narrow emission spectra allow for minimal compensation when using a single excitation source.

Immunophenotyping Scientific Posters