Flow cytometry, in conjunction with modeling algorithms, provides a powerful tool to assess cells in G0/G1 phase versus S phase, G2, or polyploidy. Life Technologies™ offers a series of fluorescent dyes to allow accurate cell cycle analysis in either live or fixed cell populations.

Select cell cycle products

See also imaging assays for live-cell cell cycle anaysis

Live cell cycle assays

The Vybrant® DyeCycle™ family of dyes offers robust fluorescent dyes for live cell cycle analysis with limited cytotoxicity using 405, 488, 532, or 633 nm excitation. The dyes have low cytotoxicity, allowing stained cells to be sorted and otherwise cultured or assessed with functional assays after staining.


Cell cycle analysis using Vybrant® DyeCycle™ Violet stain. Histogram of live Jurkat cells stained with Vybrant® DyeCycle™ Violet stain showing DNA content distribution. G0/G1 and G2/M peaks are separated by the S phase distribution. Violet 405 nm excitation was used with a 440/40 nm bandpass filter.

 

Fixed cell cycle assays

Analyzing cell cycle using FxCycle™ Violet, SYTOX® AADvanced™, or FxCycle™ Far Red stains allows for multiple color options for simplified fixed cell cycle analysis.

 
Multiparametric cell cycle and immunophenotypic analysis. TF-1 erythroblast cells were alcohol-fixed overnight, washed, and then suspended in 0.1% Triton® X-100/PBS/1% BSA before staining with anti–histone H3[pS10] purified antibody complexed with Zenon® Alexa Fluor® 488 Rabbit IgG labeling reagent and FxCycle™ Violet stain. The pH3 signal (red) identifies cells that are in mitosis.

 

Selection guides for cell cycle analysis products

  Hoechst 33342 Vybrant® DyeCycle™ Violet Vybrant® DyeCycle™ Green Vybrant® DyeCycle™ Orange Vybrant® DyeCycle™ Ruby
Basis of assay Utilize fluorescent dyes to distinguish cells in G0/G1 phase versus S phase, G2, or polyploidy
Readout DNA content analysis in living cells using a is cell permeable dye, which after binding double-stranded DNA, emits a fluorescent signal that is proportional to the DNA mass
Excitation UV or 350 UV or 405 488 488 or 532 488 or 633/5
Emission 461 437 534 563 686
Recommended filter 450/50 440/40 530/30 585/42 695/40
Multiplexable
Sample type The dyes have low cytotoxicity, allowing stained cells to be sorted and otherwise cultured or assessed with functional assays after staining.
Format 10 mL 200 assays 200 assays 200 assays 100 assays 400 assays
Cat. No. H3570 V35003 V35004 V35005 V10309 V10273
Bibliography
  Propidium Iodide ReadyProbes® Reagent FxCycle™ Violet FxCycle™ PI/RNAse Solution FxCycle™ Far Red SYTOX® AADvanced™
Basis of assay Utilize fluorescent dyes to distinguish cells in G0/G1 phase versus S phase, G2, or polyploidy
Readout DNA content analysis using a cell permeable dye, which after binding double-stranded DNA when cells are alive, emits a fluorescent signal that is proportional to the DNA mass even after fixation.
Excitation 488 or 532 405 488 or 532 633 488 or 532
Emission 617 358/461 617 660/20 695/40
Recommended filter 575/24 450/50 572/24 660/20 695/40
Multiplexable
RNase required
Included
Sample type These stains have been designed for flow cytometric analysis of DNA content in fixed cells.
Format 1 kit 1 kit 100 mL 1 kit 5 x 0.5 mL 0.2 mL
Cat. No. R37108 F10347 F10797 F10348 S10274 S10349

Cell cycle analysis scientific posters