Molecular Probes® labeling reagents enable researchers to create their own labeled biomolecule for use in immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling, and cytochemistry applications as well as for probing biological structure, function, and interactions.

Life Technologies also offers our fluorophores conjugated to a variety of antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.

Use these quick guides to select the right tools for your application or explore the resource links for more options.

Broad range of available fluorophores

We have developed an extensive catalog of fluorescent products using a range of technologies, and some of the most widely used are listed below. Which fluorophore you choose depends on the experiment you are doing and the instrument that you will use to measure the signal. find more detailed information on the fluorophore and its use by clicking on a hyperlinked dye name below.

DNA stains
  DAPI
  SYTOX® Green
  SYTO® 9
  TO-PRO®-3
  Propidium Iodide
   
   
Qdot® probes
  Qdot® 525
  Qdot® 565
  Qdot® 605
  Qdot® 655
  Qdot® 705
  Qdot® 800
   
Fluorescent protein labels
  R-Phycoerythrin (R-PE)
  Allophycocyanin (APC)
Expressed fluorescent proteins
  CFP
  GFP (emGFP)
  RFP (tagRFP)

Fluorophore dashboards

The fluorophore dashboard (sample below) allows you to visualize the performance of a selected fluorophore and determine whether it is suitable for your application and where it can be used to best effect. The dashboard provides a summary of key properties—more details are available on the product page.

  • Initial brightness indicates the relative brightness of the fluorophore, which is a product of the molar extinction coefficient and quantum yield, both of which are specific to each fluor. Fluorophores with high initial brightness can be used to detect lower-abundance targets
  • Photostability in buffer is a relative measure of the percentage of initial fluorescence intensity remaining following 30 seconds of continuous illumination using a 40x/1.4NA objective and a 100-watt Hg-arc lamp as the light source with samples mounted in phosphate buffer; high photostability in buffer is ideal for live-cell imaging and detection
  • Photostability in antifade buffer is a relative measure of the percentage of initial fluorescence intensity remaining following 30 seconds of continuous illumination using a 40x/1.4NA objective and a 100-watt Hg-arc lamp as the light source with samples mounted in ProLong® Gold or SlowFade® Gold antifade reagent; high photostability in antifade reagent is ideal for fixed-cell imaging and detection
  • Stain index is a normalized value that allows users to compare the brightness of various dyes in flow cytometry applications; it is derived by subtracting the mean fluorescence intensity (MFI) of the negative population from the MFI of the positive population and dividing the resulting value by twice the standard deviation of the negative population
  • Laser line indicates the commonly used laser line(s) for detection using flow cytometry
  • Common filter set indicates the standard microscope filter set that generates the best imaging results
  • Excitation max indicates the peak excitation wavelength (see the Fluorescence SpectraViewer for a complete profile)
  • Emission max indicates the peak emission wavelength (see the Fluorescence SpectraViewer for a complete profile)
  • Performance in SRM is a normalized value that allows users to compare the brightness of various dyes in SRM applications; SRM determinations were made in the optimum buffer type for each dye (MEA, BME, or TCEP) as described in the SRM buffer table.

Sample fluorophore dashboard


Initial brightness

 
 
 

 

 
   

 

Summary section highlighting the features of the fluorophore and its typical applications and multiplex compatibility.

 

 

Photostability in buffer

 
 
 
 
 
 
     

Photostability in antifade

 
 
 
 
 
 
   

Performance in SRM

 
 
 
 
 

  92 488 FITC 490 525  
 
Stain index

Laser line

Common filter set

Excitation max


Emission max

 

Resources

   

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.