Alexa Fluor® 405 dye
Alexa Fluor® 405 dye is a blue-fluorescent dye with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. Alexa Fluor® 405 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.
Alexa Fluor® 405 conjugates exhibit an excitation maximum at 401 nm and emission maximum at 421 nm. It is particularly useful in multicolor applications, including flow cytometry and super-resolution microscopy using dSTORM.
For imaging low-abundance targets, such as neuronal tracing in fine outgrowths, an anti–Alexa Fluor® 405 antibody is available for signal amplification. Using this anti-dye antibody in conjunction with standard enzyme-mediated immunohistochemical methods can allow you to generate a more permanent, fade-free signal for light microscopy.
We offer Alexa Fluor® 405 dye conjugated to a variety of antibodies and peptides optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.
Alexa Fluor® 405 dye dashboard
Alexa Fluor® 405 dye provides versatility in the blue channel for imaging and is compatible with the violet laser for flow cytometry. It is extremely useful in stochastic optical reconstruction microscopy (STORM) where it is used as an activator. It is also used to label conjugates for SIM.
Photostability in buffer
Photostability in antifade
Performance in SRM
Common filter set
Alexa Fluor® 405 dye products
We offer a broad range of Alexa Fluor® 405 products, including labeling kits, secondary antibodies, and bioconjugates:
Alexa Fluor® 405 conjugated to CD4, used to label 10,000 cells and optimized for signal-to-background ratio.
Alexa Fluor® 405 reactive dyes
|Amine||Aldehyde, ketone, carboxylate, or phosphate|
Alexa Fluor® 405 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
|Target protein||Kit||Cat. No.|
|Site-specific antibody labeling|
|<1–20 µg mouse IgG1||Zenon® Alexa Fluor® 405 Mouse IgG1 Labeling Kit||Z25013|
|<1–20 µg mouse IgG2a||Zenon® Alexa Fluor® 405 Mouse IgG2a Labeling Kit||Z25113|
|<1–20 µg mouse IgG2b||Zenon® Alexa Fluor® 405 Mouse IgG2b Labeling Kit||Z25213|
|<1–20 µg rabbit IgG||Zenon® Alexa Fluor® 405 Rabbit IgG Labeling Kit||Z25313|
Alexa Fluor® dye family
Illuminating research for over 15 years
Alexa Fluor® dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor® dyes.
- Learn more about the brightness of Alexa Fluor® dyes
- Learn more about the photostability of Alexa Fluor® dyes
- Read all about Alexa Fluor® dyes in the Molecular Probes® Handbook