Alexa Fluor® 532 dyeAlexa Fluor® 532 is a yellow-fluorescent dye with excitation ideally suited for the frequency-doubled Nd:YAG laser line. Alexa Fluor® 532 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in live-cell imaging applications.

Alexa Fluor® 532 conjugates exhibit an excitation maximum at 532 nm and emission maximum at 554 nm. It is particularly useful in multicolor applications, including super-resolution microscopy using dSTORM.

 

We offer Alexa Fluor® 532 dye conjugated to a variety of antibodies and peptides optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.

Alexa Fluor® 532 dye dashboard


Initial brightness


Alexa Fluor® 532 dye is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM.

   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 
   

Performance in SRM

 
 
 
 
 

  532 TRITC 532 554  
 
Laser line

Common filter set

Excitation max


Emission max

 

Alexa Fluor® 532 dye products

We offer a broad range of Alexa Fluor® 532 products including labeling kits, secondary antibodies, and bioconjugates: 

Alexa Fluor® 532 dye

Human mononuclear cells were stained with mouse anti human CD4 biotin, washed, stained with streptavidin, Alexa Fluor® 532 conjugate, washed, and analyzed by flow cytometry using 532 nm excitation.

Alexa Fluor® 532 reactive dyes

Amine Thiol

Alexa Fluor® dye family

Illuminating research for over 15 years

Alexa Fluor® dyesAlexa Fluor® dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor® dyes.

Resources

   

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.