Alexa Fluor® 594 dyeAlexa Fluor® 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor® 594 dye is pH-insensitive over a wide molar range.

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor® 594 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

We offer Alexa Fluor® 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Alexa Fluor® 594 dye dashboard


Initial brightness


Direct replacement for Texas Red® dye with bright signal and excellent photostability, especially in fixed cells; commonly used with Alexa Fluor® 350, 488, and 647 dyes for multiplexing. In addition, is used in a range of SRM applications including dSTORM, SIM, and STED and in two-photon excitation (TPE) microscopy.

   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 
   

Performance in SRM

 
 
 
 
 

  561/594 Texas Red® 590 617  
 
Laser line

Common filter set

Excitation max


Emission max

 

Alexa Fluor® 594 dye

Microtubules of fixed bovine pulmonary artery endothelial cells were visualized with mouse monoclonal anti–α-tubulin antibody, followed by an Alexa Fluor® 350 goat anti–mouse IgG antibody. Next, the F-actin was labeled with Alexa Fluor® 594 phalloidin. Finally, the cells were incubated with Alexa Fluor® 488 wheat germ agglutinin to stain components of endosomal pathways. The superimposed and pseudocolored images were acquired sequentially using bandpass filter sets appropriate for DAPI, Texas Red® dye, and fluorescein, respectively.

Alexa Fluor® 594 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Amine Thiol Aldehyde, ketone, carboxylate, or phosphate Click reaction

Alexa Fluor® 594 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Alexa Fluor® dye family

Illuminating research for over 15 years

Alexa Fluor® dyesAlexa Fluor® dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor® dyes.

Resources

   

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with Alexa Fluor® 594 dye and a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.