Alexa Fluor® 750 dyeAlexa Fluor® 750 dye is a bright, near-IR–fluorescent dye with excitation ideally suited for the 633 nm laser line or dye-pumped laser excitation. Alexa Fluor® 750 dye is pH-insensitive over a wide molar range, helping ensure that you get stable signals in imaging and flow cytometry applications.

Tandem conjugates of Alexa Fluor® 750 on primary and secondary antibodies are widely used in flow cytometry.

Alexa Fluor® 750 dye is particularly useful in stochastic optical reconstruction microscopy (STORM), where it is an exceptional reporter for both dSTORM and nSTORM.

We offer Alexa Fluor® 750 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to enable you to generate your own antibody conjugates or probes.

Alexa Fluor® 750 dye dashboard


Initial brightness

 
 
 
 
 

 


Long-wavelength emission ideal for imaging in tissues with background autofluorescence; exceptional reporter for both dSTORM and nSTORM.

 
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 
   

Performance in SRM

 
 
 
 
 

  633 Cy®7 749 775  
 
Laser line

Common filter set

Excitation max


Emission max

 

Alexa Fluor® 750
Detail of detyrosinated tubulin stained with Alexa Fluor® 750 dye shows the hollow structure generated by primary antibody labeling of microtubules. Image courtesy of Joshua Vaughan et al., University of Washington, Seattle, WA.

Alexa Fluor® 750 reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Amine Aldehyde, ketone, carboxylate or phosphate

Alexa Fluor® 750 protein/antibody labeling kits

Select an optimized labeling kit for your target antibody or protein:

Target protein Kit Cat. No.
Site-specific antibody labeling
<1–20 µg rabbit IgG Zenon® Alexa Fluor® 750 Rabbit IgG Labeling Kit Z25312
Conventional antibody/protein labeling
0.5–3 mg antibody Alexa Fluor® SAIVI™ 750 Antibody Labeling Kit S30046

Alexa Fluor® dye family

Illuminating research for over 15 years

Alexa Fluor® dyesAlexa Fluor® dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor® dyes.

Resources

   

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.