Allophycocyanin
Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.

In practical applications, the sensitivity of APC conjugates is usually 5 to 10 times greater than that of the corresponding fluorescein conjugate. In addition, RPE exhibits:

  • Long-wavelength fluorescence emission
  • Large Stokes shifts
  • Quench-resistance due to the protein backbone
  • Very high water solubility
  • Homogeneous structure with defined molecular weights

Life Technologies offers APC conjugated to a variety of primary antibodies, secondary antibodies, and streptavidin. In addition, tandem conjugates of phycobiliproteins are available to generate a range of emission signals from a single excitation wavelength.

Allophycocyanin (APC) dashboard


Initial brightness

 
 
 
 
 

 

Allophycocyanin, also known as APC, is typically used for flow cytometry, microarrays, ELISA, and other applications that require high sensitivity but not photostability.

 
   

Photostability in buffer

 
 
 
 
 

  200 594–633 Cy®5 650 660  
 
Stain index

Laser line

Common filter set

Excitation max

Emission max
 

Definition of terms, and how these values were derived

Allophycocyanin (APC) products

We offer a broad range of APC products, including primary antibodies, secondary antibodies, streptavidin conjugates, and more.

Allophycocyanin (APC)

APC Alexa Fluor® 750 conjugated to CD4, used to label 10,000 cells and optimized for signal-to-background ratio.

Resources

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Flow cytometry instrument controls and standards

Flow instrument controlsKeep your flow cytometry running at peak efficiency with Molecular Probes® instrument set-up, calibration, and counting beads including alignment and linearity beads, compensation tools, and cell-counting beads.

     

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.