Cyan Fluorescent ProteinCyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm.

Life Technologies offers a series of RFP CellLight® fusion constructs of signal peptides or cell structure proteins with CFP for accurate and specific targeting to subcellular structures, including the nucleus and plasma membrane.

CellLight® reagents offer high spatial and temporal resolution for live-cell applications and tolerate fixation with formaldehyde for fixed-cell analysis. Green Fluorescent Protein, Red Fluorescent Protein, and Cyan Fluorescent Protein constructs are available for multiplexing with cellular stains and other CellLight® reagents.

Cyan Fluorescent Protein (CFP) dashboard


Initial brightness

 
 
 
 
 

 


Cyan Fluorescent Protein is a useful expression label in the blue channel for flow cytometry or imaging applications; the signal is unchanged in antifade reagent or buffer. 

 
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 

  350, 405 DAPI 435 485  
 
Laser line

Common filter set

Excitation max


Emission max

 

Definition of terms, and how these values were derived

CFP products

CellLight® reagents

CellLight® reagents are ready-to-use fluorescent protein constructs targeted to specific subcellular structures. These reagents provide a simple and effective method for introducing targeted intracellular labels within living cells. Simply add the reagent to your cells, incubate overnight, and you’re ready to image your cells.

 
Cyan Fluorescent Protein

Live cell imaging with CellLight™ reagents. HEK-293 cells transduced with CellLight™ Plasma Membrane-CFP and CellLight™ Peroxisome-GFP. The following day, live-cell imaging was performed using a 60x objective on a DeltaVision® Core microscope and standard DAPI/FITC/TRITC filter sets.

BacMam technology

  • Efficient transduction of cell lines, including primary cells and stem cells
  • Safe transduction and lack of observable cytopathic effects
  • Easy modulation of gene expression
  • Expression of multi-component complexes at the right stoichiometry

The BacMam technology is based on double-stranded DNA insect viruses (baculovirus) as vehicles to efficiently deliver and express genes in mammalian cells, including those encoding fluorescent proteins. such as Cyan Fluorescent Protein. Baculoviruses are well-tolerated and nonreplicating in mammalian cells, so they have an excellent safety profile for routine applications.

Learn more about:

 

Bacmam technology

Live-cell imaging of HeLa cells using CellLight™ mitochondria and talin probes, transduced using BacMam 2.0 technology.

Resources

   

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.

     

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.