Oregon Green® 488 dye
Oregon Green® 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. It has a high extinction coefficient and fluorescence quantum yield and while it is pH insensitive in the physiological range, with a pKa of 4.6 it can be used to monitor pH change in acidic organelles or pathways.

Oregon Green® 488 dye forms the base of a number of ion indicators used for physiological analysis, including calcium.

We offer Oregon Green® 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own protein conjugates or probes.

Oregon Green® 488 dashboard


Initial brightness

 
 
 
 
 

 

Oregon Green® 488 dye is a green fluorescent dye that is used to label a number of protein conjugates and lipid probes; it also forms the basis for a series of ion indicators.

 
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 

  488 FITC 501 526  
 
Laser line

Common filter set

Excitation max


Emission max

 

Definition of terms, and how these values were derived

Oregon Green® 488 dye products

We offer a broad range of Oregon Green ® 488 dye products including labeling kits, secondary antibodies, bioconjugates, and more.

Learn more here:

Oregon Green® 488 dye signals can be amplified with anti-dye antibodies.

Oregon Green® 488 dye

Microtubules visualized with Oregon Green® 488 paclitaxel.

Microtubules were assembled, stabilized and visualized with the aid of green-fluorescent Oregon Green® 488 paclitaxel. The image was acquired using a confocal laser-scanning microscope and a filter set appropriate for fluorescein.

Oregon Green® 488 dye reactive dyes

Select dye-labeling chemistries for conjugation with the following reactive groups: 

Amine Thiol Aldehyde-, ketone, or carboxylate Alkyne terminated

High-performance alternatives to Oregon Green® 488 dye

Alexa Fluor® 488 dye

Alexa Fluor® 488 dye is a bright, green-fluorescent dye with excitation and emission wavelengths similar to those of Oregon Green® 488 dye. For stable signal generation in imaging and flow cytometry, Alexa Fluor® 488 dye is pH-insensitive over a wide concentration range.

Monitoring pH in acidic organelles

For pH monitoring, the pHrodo™ green dye is almost nonfluorescent at neutral pH and fluoresces brightly in acidic environments. Unlike fluorescein, the minimal dye fluorescence at neutral pH also eliminates the need for wash steps and quencher dyes because any non-internalized dye will be essentially nonfluorescent.

Monitoring intracellular calcium flux

Calcium flux assays are widely used for in-cell determination of agonist-stimulated signaling pathways and functional neuronal measurement. Oregon Green® probes emit green fluorescence at resting levels of Ca2+ and increase their fluorescence intensity up to 14-fold with increasing Ca2+ concentration. The fluo series of calcium indicators emits minimal fluorescence at resting levels of Ca2+, and each increases its fluorescence intensity up to >100-fold with increasing Ca2+ concentration.

Resources

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with Oregon Green® 488 dye and a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.

     

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.