Qdot® 525 Probe
Qdot® 525 probe is an excellent green-fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~525 nm, Qdot® 525 probe can be excited using a 488 nm laser line or standard FITC filter. Optimum imaging results with brighter signals are achieved with a Qdot® 525 filter or light cube.
Made from nanocrystals of semiconductor material, Qdot® probes are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. In general they have an extremely broad excitation range and narrow emission with a large Stokes shift. Optimal brightness is generally achieved using a specific Qdot® filter.
Organic fluorophores and Qdot® probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot® probes are also ideal for multiplexed western blots.
Qdot® 525 probe dashboard
For best results use a Qdot®-optimized filter and PBS or a mounting medium without antifade.
Photostability in buffer
Common filter set
Qdot® 525 products
The exceptional brightness and photostability of Qdot® probes make it easier to detect low-abundance targets. With narrow and symmetric emission spectra, Qdot® probes are also ideal for multiplexed fluorescence detection using a combination of Qdot® probes and organic dyes.
- Qdot® 525 streptavidin conjugates
- Qdot® 525 secondary antibody conjugates
- SiteClick™ Qdot® 525 Antibody Labeling Kit
- Qtracker® 525 Cell Labeling Kit
Qdot® 525 conjugation kits
We’ve developed functionalized versions of the Qdot® 525 label for the preparation of custom conjugates of proteins and other biomolecules. The first—a carboxyl-derivatized form—can be coupled to amine groups of proteins and modified oligonucleotides. The second—an amine-derivatized PEG form—can be coupled to isothiocyanates and succinimidyl esters (or with native carboxylic acids using water-soluble carbodiimides).
|Amine||Aldehyde-, ketone, carboxylate or phosphate|
Qdot® probes and accessories
Qdot® Probes are made from nanocrystals of semiconductor material and are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. Learn more about Qdot® Probes:
- Qdot® probes overview
- Qdot® probe technology
- Qdot® probes structure
- Qdot® probes size
- EVOS® Light Cube, Qdot® 565
Designed to eliminate the complexities of microscopy without compromising performance. The Qdot® 565 light cube is ideal for use with Qdot® 565 and other fluorophores with excitation and emission maxima near 445 and 565 nm, respectively.
- See a complete list of Qdot® products