Qdot® 605 Probe
Qdot® 605 probe is an excellent red-fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~605 nm, Qdot® 605 probe can be excited using 350, 488, 561, or 594 nm laser lines or standard TRITC filter. Optimum imaging results with brighter signals are achieved with a Qdot® 605 filter or light cube.
Made from nanocrystals of semiconductor material, Qdot® probes are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. In general they have an extremely broad excitation range and narrow emission with a large Stokes shift. Optimal brightness is generally achieved using a specific Qdot® filter.
Organic fluorophores and Qdot® probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot® probes are also ideal for multiplexed western blots.
Qdot® 605 probe dashboard
For best results use a Qdot®-optimized filter and PBS or a mounting medium without antifade.
Photostability in buffer
|35||350, 488, 561, 594||RFP||UV||605|
Common filter set
Qdot® 605 products
The exceptional brightness and photostability of Qdot® probes make it easier to detect low-abundance targets. With narrow and symmetric emission spectra, Qdot® probes are also ideal for multiplexed fluorescence detection using a combination of Qdot® probes and organic dyes.
- Qdot® 605 streptavidin
- Biotin-labeled Qdot® 605 probes
- Qdot® 605 secondary antibody conjugates
- Qdot® 605 primary antibody conjugates
- Isotype antibody controls - Mouse IgG2a, Qdot® 605 conjugate
- Antibody labeling - Qdot® 605 Antibody Conjugation Kit and the SiteClick™ Qdot® 605 Antibody Labeling Kit
- Qtracker® 605 Cell Labeling Kit
Flow cytometric analysis of whole blood cells using streptavidin conjugates of Qdot® nanocrystals.
Ammonium chloride lysed whole blood cells were stained with mouse anti human CD3 biotin, washed, stained with Qdot® 605 streptavidin conjugate, washed, stained with mouse anti human CD56 biotin, washed, and stained with Qdot® 705 streptavidin conjugate, washed, resuspended, and analyzed by flow cytometry using 405 nm excitation and detected using 605⁄20 nm and 710⁄20 nm emission bandpass filters. The dot plot shows Qdot® 705 streptavidin conjugate on the Y-axis and Qdot® 605 streptavidin conjugate on the X-axis.
Qdot® 605 conjugation kits
We’ve developed functionalized versions of the Qdot® 605 label for the preparation of custom conjugates of proteins and other biomolecules. The first—a carboxyl-derivatized form—can be coupled to amine groups of proteins and modified oligonucleotides. The second—an amine-derivatized PEG form—can be coupled to isothiocyanates and succinimidyl esters (or with native carboxylic acids using water-soluble carbodiimides).
|Amine||Aldehyde-, ketone, carboxylate or phosphate|
Qdot® probes and accessories
Qdot® Probes are made from nanocrystals of semiconductor material and are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. Learn more about Qdot® Probes:
- Qdot® probes overview
- Qdot® probe technology
- Qdot® probes structure
- Qdot® probes size
- EVOS® Light Cube, Qdot® 565
Designed to eliminate the complexities of microscopy without compromising performance. The Qdot® 565 light cube is ideal for use with Qdot® 565 and other fluorophores with excitation and emission maxima near 445 and 565 nm, respectively.
- See a complete list of Qdot® products