R-phycoerythrin (R-PE)
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability.

In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. In addition, R-PE exhibits:

  • Long-wavelength fluorescence emission
  • Large Stokes shifts
  • Quench resistance due to the protein backbone
  • Very high water solubility
  • Homogeneous structure with defined molecular weights

Life Technologies offers R-PE conjugated to a variety of primary antibodies, secondary antibodies, and streptavidin. In addition, tandem conjugates of phycobiliproteins are available to generate a range of emission signals from a single excitation wavelength.

Phycoerythrin (R-PE) dashboard


Initial brightness

 
 
 
 
 

 

Phycoerythrin, also known as R-PE, is typically used for flow cytometry, microarrays, ELISA, and other applications that require high sensitivity but not photostability.

 
   

Photostability in buffer

 
 
 
 
 

  158 488–561 TRITC 565 578  
 
Stain index

Laser line

Common filter set

Excitation max

Emission max
 

R-phycoerythrin (R-PE) products

We offer a broad range of RPE products, including primary antibodies, secondary antibodies, streptavidin conjugates, and more.

R phycoerythrin (R-PE)

Flow cytometry histogram showing human mononuclear cells stained with anti-CD4 complexed with R-phycoerythrin Zenon® labeling reagent.

Resources

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Flow cytometry instrument controls and standards

Flow instrument controlsKeep your flow cytometry running at peak efficiency with Molecular Probes® instrument set-up, calibration, and counting beads including alignment and linearity beads, compensation tools, and cell-counting beads.

     

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.