SYTO® 9 Stain

SYTO® 9 stain is an excellent green-fluorescent nuclear and chromosome counterstain that is permeant to both prokaryotic and eukaryotic cell membranes. SYTO® 9 stain has a high affinity for DNA and exhibits enhanced fluorescence upon binding with an excitation maximum at 483 nm and fluorescence emission maximum at 503 nm.

SYTO® 9 stain is particularly useful as a nuclear counterstain for bacterial assays since it stains both live and dead Gram-positive and Gram-negative bacteria.

SYTO® 9 stain dashboard


Initial brightness

 
 
 
 
 

 


Live-cell SYTO® dyes are permeant to both eukaryotic and prokaryotic membranes, and exhibit enhanced fluorescence upon binding to both DNA and RNA.

 
   

Photostability in buffer

 
 
 
 
 
   

Photostability in antifade

 
 
 
 
 

  488 FITC 483 503  
 
Laser line

Common filter set

Excitation max


Emission max

 

Definition of terms, and how these values were derived

SYTO® 9 stain products

The SYTO® green-fluorescent nucleic acid stains have proven valuable in a broad range of research applications. SYTO® 9 stain is particularly used for staining live and dead Gram-positive and Gram-negative bacteria.

SYTO® 9 stain

Live/dead analysis of Streptomyces antibioticus ETH7451 cultures.

The mycelium was stained with SYTO® 9 dye and propidium iodide (components of the LIVE/DEAD® BacLight™ Bacterial Viability Kit, Cat. Nos. L7007, L7012, L13152). Live portions fluoresce green, and membrane-compromised portions fluoresce red. Image contributed by Jesus Sanchez and Marisol Fernandez, Microbiology Area, University of Oviedo, Spain, and reproduced with the permission of Microbiology 148, 405 (2002)

High-performance nucleic acid stains

Unlike our range of nuclear counterstains, SYTO® dyes do not act exclusively as nuclear stains in live cells and should not be equated with DNA-selective compounds (such as DAPI) which stain nuclei in live animal cells. Eukaryotic cells incubated with SYTO® dyes generally show cytoplasmic or mitochondrial staining, as well as nuclear staining.

Nuclear counterstains provide highly selective nuclear staining with little or no cytoplasmic labeling and a choice of colors for multiplexing with other labels. Additionally, some of the stains can also be used in cell cycle analyses using high-content imaging and flow cytometry.

 

FluoCells prepared slide #6 showing a fixed, permeabilized, and labeled muntjac skin fibroblast.

Mitochondria were labeled with mouse anti–OxPhos Complex V inhibitor protein antibody and visualized using orange-fluorescent Alexa Fluor® 555 goat anti–mouse IgG antibody. F-actin was labeled with green-fluorescent Alexa Fluor® 488 phalloidin, and the nucleus was stained with TO-PRO®-3 iodide (pseudocolored magenta).

 

Resources

Molecular Probes® Handbook

Molecular Probes HandbookThe significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes® Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.

 

Antifade reagents

Antifade reagentsAntifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with SYTO® 9 stain and a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications.

     

Cell stain tool

Cell stain toolStain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.

 

Fluorescence SpectraViewer

Fluorescence SpectraviewerPlot and compare spectra and check the spectral compatibility for common fluorophores including all the Molecular Probes® fluorescent probes. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.

For Research Use Only. Not for use in diagnostic procedures.