Click-iT® Assay Kits   Click-iT® technology provides a streamlined and simplified workflow for multiple applications in microscope imaging, flow cytometry, and high-content analysis or screening. We’ve developed a series of optimized assay kits designed to generate reliable results, including kits to measure cell proliferation, new protein synthesis, RNA synthesis, lipid peroxidation, and apoptosis using TUNEL assays. Additionally, Click-iT® technology can be used as a sensitive tool for molecular capture, and we offer kits to capture nascent proteins and RNA. The topics below will assist you in selecting the right kit based on your assay platform and application.

Click-iT® Assay Kits

Assay kits for imaging

Click-iT® Assay Kits for imaging provide a robust workflow with a selection of detection options to allow for multiplexing and provide a broader biological context for your results. Imaging kits are optimized for microscopy and offered with a range of Alexa Fluor® dyes for detection.

Assay kits for HCS

Click-iT® HCS Assay kits are optimized for higher throughput and provide reagent for multiple microplates in each kit. Not all imaging kits are provided in a high throughput format.

 
Click-iT® Plus EdU Alexa Fluor® 488 Imaging KitProliferating HeLa cells detected using the Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit.

Assay kits for flow cytometry

Click-iT® Assay Kits for Flow Cytometry provide the most convenient to measure cell proliferation by tracking new DNA synthesis. Cell proliferation assays are offered with a range of Alexa Fluor® dyes for detection to allow for multiplexing with common fluorophores, including R-PE and R-PE tandems, as well as fluorescent proteins.

Kits for biomolecule capture

When Click-iT® technology is used for molecular capture, it enables the enrichment of newly synthesized RNA or protein species that can be used in subsequent gene expression analysis or protein sequencing.

  Click-iT® Plus EdU Alexa Fluor® 488 Kit

Compatibility of Click-iT® Plus EdU reaction with R-PE tandem conjugate (R-PE Cy®7) fluorescence.

Ordering information

Product Quantity Cat. No.
Click-iT® kits for protein analysis
Click-iT® HPG Alexa Fluor® 488 Protein Synthesis Assay Kit 1 kit C10428
Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit 1 kit C10429
Click-iT® Plus OPP Alexa Fluor® 488 Protein Synthesis Assay Kit 1 kit C10456
Click-iT® Plus OPP Alexa Fluor® 594 Protein Synthesis Assay Kit 1 kit C10457
Click-iT® Plus OPP Alexa Fluor® 647 Protein Synthesis Assay Kit 1 kit C10458
Click-iT® kits for cell proliferation
Click-iT® EdU Alexa Fluor® 488 Imaging Kit 1 kit, 50 to 250 coverslips C10337
Click-iT® EdU Alexa Fluor® 555 Imaging Kit 1 kit, 50 to 250 coverslips C10338
Click-iT® EdU Alexa Fluor® 594 Imaging Kit 1 kit, 50 to 250 coverslips C10339
Click-iT® EdU Alexa Fluor® 647 Imaging Kit 1 kit, 50 to 250 coverslips C10340
Click-iT® Plus EdU Alexa Fluor® 488 Imaging Kit 1 kit, 50 to 250 coverslips C10637
Click-iT® Plus EdU Alexa Fluor® 555 Imaging Kit 1 kit, 50 to 250 coverslips C10638
Click-iT® Plus EdU Alexa Fluor® 594 Imaging Kit 1 kit, 50 to 250 coverslips C10639
Click-iT® Plus EdU Alexa Fluor® 647 Imaging Kit 1 kit, 50 to 250 coverslips C10640
Click-iT® kits for TUNEL assays
Click-iT® TUNEL Alexa Fluor® 488 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10245
Click-iT® TUNEL Alexa Fluor® 594 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10246
Click-iT® TUNEL Alexa Fluor® 647 Imaging Assay, for microscopy & HCS 1 kit (1 microplate or 50 coverslips) C10247
Click-iT® kits for RNA synthesis assays
Click-iT® RNA Alexa Fluor® 488 Imaging Kit 1 kit C10329
Click-iT® RNA Alexa Fluor® 594 Imaging Kit 1 kit C10330
Click-iT® kits for lipid peroxidation assays
Click-iT® Lipid Peroxidation Imaging Kit, Alexa Fluor® 488 1 kit, 500 assays C10446
Product Quantity Cat. No.
Click-iT® kits for nascent protein capture
Click-iT® Protein Enrichment Kit, for click chemistry capture of azide-modified proteins 1 kit C10416
Click-iT® kits for nascent RNA capture
Click-iT® Nascent RNA Capture Kit, for gene expression analysis 1 kit C10365