DMEM, RPMI, and MEM

Reproducible results at a lower cost

Reproducible results

  • Fewer lot-to-lot changes of serum means less variability in your cell culture conditions

Lower cost

  • Using less serum and testing fewer lots results in reduced expenses for your laboratory

Order Now

Equivalent Growth Using Less Sera

GIBCO® Advanced™ media are enhanced basal media formulations of DMEM, DMEM/F-12, MEM, and RPMI 1640.  Enriched with normal-serum constituents, these media require 50–90% less FBS supplementation, with equivalent or superior cell growth, and no change in morphology or function of many common cell lines (Figure 1).

For many standard cell lines, Advanced™ media can be used as a direct replacement for classical media.  Growth comparisons of several cell lines grown in Advanced™ media (1–2% FBS) compared to classical media (5% FBS) show equivalent or superior cell growth and no change in morphology (Figure 1).

RPMI Chart

Figure 1. Growth comparison of multiple cell lines in standard RPMI 1640 supplemented with 5% FBS to Advanced™ RPMI 1640 supplemented with 0.5% to 5% FBS.


Standard RPMI 1640 supplemented with 4 mM L-glutamine and 5% FBS was compared to Advanced™ RPMI 1640 supplemented with 4 mM L-glutamine and 5%, 2%, 1%, or 0.5% FBS. SP2, Jurkat, VERO, Raji, and Daudi cells were plated at an appropriate seeding density per 25-cm2 flask and incubated at 37˚C in a 5% CO2 and 95% air-humidified incubator over a four-day passage cycle for two passages.  On the third passage, cells were seeded appropriately into 24-well plates and incubated as noted above for seven days.  Average cell count at day 5 (triplicate samples) is reported above.

Recommended Supplementation for Tested Cell Lines

Equivalent Growth Using Less Sera

GIBCO® Advanced™ media are enhanced basal media formulations of DMEM, DMEM/F-12, MEM, and RPMI 1640.  Enriched with normal-serum constituents, these media require 50–90% less FBS supplementation, with equivalent or superior cell growth, and no change in morphology or function of many common cell lines (Figure 1).

For many standard cell lines, Advanced™ media can be used as a direct replacement for classical media.  Growth comparisons of several cell lines grown in Advanced™ media (1–2% FBS) compared to classical media (5% FBS) show equivalent or superior cell growth and no change in morphology (Figure 1).

RPMI Chart

Figure 1. Growth comparison of multiple cell lines in standard RPMI 1640 supplemented with 5% FBS to Advanced™ RPMI 1640 supplemented with 0.5% to 5% FBS.

Standard RPMI 1640 supplemented with 4 mM L-glutamine and 5% FBS was compared to Advanced™ RPMI 1640 supplemented with 4 mM L-glutamine and 5%, 2%, 1%, or 0.5% FBS. SP2, Jurkat, VERO, Raji, and Daudi cells were plated at an appropriate seeding density per 25-cm2 flask and incubated at 37˚C in a 5% CO2 and 95% air-humidified incubator over a four-day passage cycle for two passages.  On the third passage, cells were seeded appropriately into 24-well plates and incubated as noted above for seven days.  Average cell count at day 5 (triplicate samples) is reported above.

Recommended Supplementation for Tested Cell Lines

Other Benefits of Advanced™ Media

Direct substitution for basal media

For most applications, no weaning or adaptation procedures are necessary to reduce serum supplementation by at least 50% in a wide variety of cell lines.  Provided in a 1x liquid format, Advanced™ DMEM, DMEM/F-12, MEM, and RPMI 1640 only require the addition of GlutaMAX™-I Supplement or L-glutamine and the appropriate reduced amount of serum to make complete media.

Using less sera has multiple benefits

Lowering your use of serum addresses many issues of cost, supply, quality, consistency, and regulatory concerns that you face daily in cell culture.

Advanced™ media is a trusted GIBCO® product

GIBCO® products are infused with quality, customer-focused innovation, and service excellence from beginning to end.  GIBCO® products always provide the highest quality, consistency and performance - for results that you can count on every day.  The widest range of cell culture products, award-winning service, and proven performance - GIBCO® products provide you with the quality and consistency you depend upon.  When reproducible results matter, use GIBCO® products.

Ordering Information

Sku Name Size Price Qty
12491015 Advanced DMEM 500 mL USD 29.00
12491023 Advanced DMEM 10 x 500 mL USD 251.00
12492013 Advanced MEM 500 mL USD 29.00
12492021 Advanced MEM 10 x 500 mL USD 251.00
12633012 Advanced RPMI 1640 Medium 500 mL USD 28.90
12633020 Advanced RPMI 1640 Medium 10 x 500 mL USD 251.00
12634010 Advanced DMEM/F-12 500 mL USD 41.60
12634028 Advanced DMEM/F-12 10 x 500 mL USD 362.00
35050061 GlutaMAX™ Supplement 100 mL USD 30.40
12605010 TrypLE™ Express Enzyme (1X), phenol red 100 mL USD 21.90