TrypLE Express

TrypLE™ Express (1X), TrypLE™ Select (1X), and TrypLE™ Select (10X) reagents

  • Gentle on cells—maintains cell health for reproducible results
  • Saves time—achieves faster dissociation for a wide variety of cells
  • Animal origin-free—pure, consistent and effective
  • Room-temperature stable—more convenient to use

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TrypLE™ Express—uses significant economies of scale to make TrypLE easily accessible to all researchers

TrypLE™ Select (1X)—manufactured on dedicated animal origin-free equipment

TrypLE™ Select (10X)—for fast dissociation of strongly adherent cells and manufactured on dedicated animal origin-free equipment

Gentle and convenient
TrypLE™ reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE™ reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol.

Stable at room temperature

TrypLE™ reagent is room-temperature stable and ready to use when you need it. TrypLE™ cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

TrypLE™ Express reagent

TrypLE™ Express reagent proves that superior performance can be affordable. Significant economies of scale make it even easier to replace trypsin with TrypLE™ Express reagent. For fast, effective cell dissociation that doesn’t harm your cells, use TrypLE™ Express reagent.

TrypLE™ Select (1X) reagent

TrypLE™ Select (1X) reagent is manufactured on dedicated animal origin–free equipment in our state-of-the art facilities in Grand Island, New York.

Cell release times with TrypLE™ Select (1X) reagent

TrypLE™ Select
Cell line Media Mean time required
for cell release (min)
Mean viability (%)
293F DMEM + 5% FBS 2 97
VERO VP-SFM 5 100
VERO EMEM + 5% FBS 8 100
VERO OptiPRO™ SFM 7 98
CHO-K1 CHO III A 7 95

TrypLE™ Select (10X) reagent

TrypLE™ Select (10X) reagent can be used at full strength to quickly and gently dissociate strongly adherent cell types. TrypLE™ Select (10X) reagent is also manufactured on dedicated animal origin–free equipment in our state-of-the art facilities in Grand Island, New York. 

Strongly adherent cell release times

TrypLE™ Select 10x TrypLE™ Select
Strongly adherent
cell lines
Media Mean time required
for cell release (min)
Mean
viability (%)
Mean time required
for cell release (min)
Mean
viability (%)
PK-15 EMEM + 10% FBS 11.7 ± 2.0 99.4 ± 0.3 27 98
PK-15 OptiPRO™ SFM N/A N/A 11 99
MDCK OptiPRO™ SFM 15.3 ± 0.8 98.7 ± 0.5 28 98
MDBK DMEM + 5% FBS 8.0 ± 1.3 98.8 ± 0.5 15 100
A549 DMEM + 10% FBS 5.2 ± 0.3 99.1 ± 0.5 9 98
MSC DMEM + 10% MSC FBS 7.8 ± 1.2 98.7 ± 1.3 N/A N/A
MSC StemPro® MSC SFM 8.7 ± 1.5 97.5 ± 0.1 N/A N/A