Gibco® trypsin solutions are used widely for dissociation of tissues and cell monolayers.

The biochemical assays performed on Trypsin 1:250 determine both trypsin-specific activity at the level of certain co-purified enzymes that influence cell removal and viability. All trypsin solutions are prepared from porcine parvovirus-tested and mycoplasma-tested materials.

Gibco® trypsin solutions are prepared under controlled conditions using accelerated manufacturing procedures, to minimize loss of enzyme activity from denaturation and autodegradation. All raw stock trypsin are verified for e-beam irradiation and tested for PPV, mycoplasma, and PCV 1/2 contamination.

Gibco® trypsin products will ship on gel ice starting November 1, 2014. Find out why

Ordering Information

TrypLE™ Express: Trypsin Replacement Enzyme

TrypLE™ Express:  the superior replacement for trypsin

  • Gentle on cells - higher plating efficiency
  • Saves time - eliminates the need to stagger harvesting
  • Stable at room temperature  - ready to use when you need it
  • Easy to use - directly substitutes into existing protocols

Choose the Proper Cell Dissociation Product

Culture Type Wash Solution Dissociation Solution
Products and Cat. No.
Most continuous and strongly adherent cell lines

Many cells at early passage
HBSS or PBS w/o calcium and magnesium

D-PBS
Trypsin 0.25% in balanced salt solution w/o calcium and magnesium

TrypLE™ products
0.25% Trypsin (liquid) (1X) 15050-065
2.5% Trypsin (liquid) (10X) 15090-046
Trypsin Powder (1:250) 27250-018

TrypLE™ Select (liquid) 12563-011
TrypLE™ Express (liquid) 12605-010
Continuous cell lines where cell surface protein integrity is important HBSS or PBS w/o calcium and magnesium Trypsin 0.05% in 0.53 mM EDTA



TrypLE™ products
0.05% Trypsin 0.53 mM EDTA (liquid) 25300-054
0.05% Trypsin 0.53 mM EDTA (liquid) (10X) 15400-054

TrypLE™ Select (liquid) 12563-011
TrypLE™ Express (liquid) 12605-010
Weakly adherent epithelial cells


Transformed fibroblasts

Primary cells where cell surface protein integrity is important
HBSS or PBS w/o calcium and magnesium EDTA, glycerol in sodium citrate


TrypLE™ products
Enzyme-Free Cell Dissociation Buffers, Hanks' based 13150-016, PBS based 13151-014

TrypLE™ Select (liquid) 12563-011
TrypLE™ Express (liquid) 12605-010
Strongly adherent early-passage cell lines HBSS or PBS w/o calcium and magnesium Trypsin 0.25% in 1 mM EDTA


Dispase 0.6 to 2.4 units/ml in PBS
0.25% Trypsin 1 mM EDTA (liquid) 25200-056
Trypsin Powder (1:250) 27250-018

Dispase
Epithelial cells 0.5 mM to 1 mM EDTA 0.5 mM to 1 mM EDTA


Dispase 0.6 to 2.4 units/ml in PBS
Versene 1:5,000 (0.53 mM EDTA in PBS)

Dispase
Strongly adherent, epithelial and some tumor cell lines
0.5 mM to 1 mM EDTA 0.25% with 1 mM EDTA





Dispase 0.6 to 2.4 units/ml in PBS w/o calcium and magnesium
0.25% Trypsin 1 mM EDTA (liquid) 25200-056
Versene 1:5,000 (0.53 mM EDTA in PBS)
Trypsin Powder (1:250) 27250-018

Dispase
Thick cultures, multiple layers


Dense cultures that are collagen rich
1 mM EDTA Trypsin 0.25%



Collagenase 200 units/ml, 1 mM EDTA in balanced salt solution w/o calcium and magnesium
0.25% Trypsin 1 mM EDTA (liquid) 25200-056
Trypsin Powder (1:250) 27250-018

Collagenase Type I, Type II, Type IV


All adherent cell cultures HBSS or PBS w/o calcium and magnesium Scrape off cell sheet