Keratinocyte Cell Culture

Our Primary Human Keratinocyte Cell Cultures are optimized to synthesize Keratinocyte Cells, the predominant cell type in the epidermis. The Keratinocytes found in the basal layer or stratum germinativum of the skin are sometimes referred to as basal cells or basal Keratinocytes.

Primary Human Keratinocyte Cells adult (HEKa)
Primary Human Keratinocyte Cells neonatal (HEKn)

  • > 500,000 cells/vial cryopreserved at the end of the 1' culture
  • > 70% viability
  • > 25 population doublings HEKa (adult)
  • > 30 population doublings HEKn (neonatal)
  • Optimized for superior performance over the leading competitors

Looking for more information?  Checkout our Introduction to Cutaneous Biology!

For a comprehensive list of cells, technical resources and related technologies visit the Cell Resource Center

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Phase contrast images of Human Epidermal Keratinocytes (HEKa) grown in culture

HEKa cells grown in EpiLife (M-EPI-500-CA) with the addition of Human Keratinocyte Growth Supplement (HKGS). First culture after thaw.
Keratinocyte Cell Culture
Day 3 - HEKa

Keratinocyte Cell
Day 5 - HEKa

Keratinocyte Cells
Day 7 - HEKa

Get more life out of you cells

GIBCO EpiLife serum-free cell culture medium
  • Defined, animal origin-free medium
  • Extends the in vitro lifespan of primary cells up to two times compared to other serum-free media
  • Ease of use with single-shot supplementation
Keratinocyte Cell Culture Graph




Figure 1
- Normal neonatal human keratinocytes (HEKn) (C-001-5C) were grown in EpiLife medium and in a keratinocyte medium from a leading competitor. Cultures grown in EpiLife medium demonstrated population doublings over an extended period compared to cells grown in the competitor's medium.

Keratinocyte Product Selection Guide

If you'd like to culture your cells....

  • in an animal-product free (APF) environment
  • for an extended lifespan




  • in a chemically-defined environment
  • for an extended lifespan



  • in a Bovine Pituitary Extract (BPE) containing environment
  • for an extended lifespan



  • in a Bovine Pituitary Extract (BPE) containing environment
  • for a standard lifespan
Then choose: