Keratinocyte Cell Culture

Our Primary Human Keratinocyte Cell Cultures are optimized to synthesize Keratinocyte Cells, the predominant cell type in the epidermis. The Keratinocytes found in the basal layer or stratum germinativum of the skin are sometimes referred to as basal cells or basal Keratinocytes.

Primary Human Keratinocyte Cells adult (HEKa)
Primary Human Keratinocyte Cells neonatal (HEKn)

  • > 500,000 cells/vial cryopreserved at the end of the 1' culture
  • > 70% viability
  • > 25 population doublings HEKa (adult)
  • > 30 population doublings HEKn (neonatal)
  • Optimized for superior performance over the leading competitors

Looking for more information?  Checkout our Introduction to Cutaneous Biology!

For a comprehensive list of cells, technical resources and related technologies visit the Cell Resource Center

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Phase contrast images of Human Epidermal Keratinocytes (HEKa) grown in culture

HEKa cells grown in EpiLife (M-EPI-500-CA) with the addition of Human Keratinocyte Growth Supplement (HKGS). First culture after thaw.
Keratinocyte Cell Culture
Day 3 - HEKa

Keratinocyte Cell
Day 5 - HEKa

Keratinocyte Cells
Day 7 - HEKa

Get more life out of you cells

GIBCO EpiLife serum-free cell culture medium
  • Defined, animal origin-free medium
  • Extends the in vitro lifespan of primary cells up to two times compared to other serum-free media
  • Ease of use with single-shot supplementation
Keratinocyte Cell Culture Graph




Figure 1
- Normal neonatal human keratinocytes (HEKn) (C-001-5C) were grown in EpiLife medium and in a keratinocyte medium from a leading competitor. Cultures grown in EpiLife medium demonstrated population doublings over an extended period compared to cells grown in the competitor's medium.

Keratinocyte Product Selection Guide

If you'd like to culture your cells....

  • in an animal-product free (APF) environment
  • for an extended lifespan




  • in a chemically-defined environment
  • for an extended lifespan



  • in a Bovine Pituitary Extract (BPE) containing environment
  • for an extended lifespan



  • in a Bovine Pituitary Extract (BPE) containing environment
  • for a standard lifespan
Then choose:



Ordering Information

Sku Name Size Price Qty
C0055C Human Epidermal Keratinocytes, adult (HEKa) 1 vial USD 387.00
C0215C Human Epidermal Keratinocytes, adult, Animal Product-Free (HEKa-APF) 1 vial USD 453.00
C0015C Human Epidermal Keratinocytes, neonatal (HEKn) 1 vial USD 402.00
C0205C Human Epidermal Keratinocytes, neonatal, Animal Product-Free (HEKn-APF) 1 vial USD 464.00
MEPICF500 EpiLife® CF Kit 500 mL USD 76.00
MEPICFPRF500 EpiLife® CF/PRF Kit 500 mL USD 77.00
MEPI500CA EpiLife® Medium, with 60 µM calcium 500 mL USD 70.00
A1051501 Keratinocyte AOF Growth Kit 1 kit USD 165.00
M154500 Medium 154 500 mL USD 60.00
M154CF500 Medium 154CF, Kit 500 mL USD 67.00
M154CFPRF500 Medium 154CF/PRF, Kit 500 mL USD 67.00
R01510 Gentamicin/Amphotericin Solution 10 x 1 mL USD 64.00
R001100 Trypsin/EDTA Solution (TE) 100 mL USD 22.69
R007100 Defined Trypsin Inhibitor 100 mL USD 35.60
R002100 Trypsin Neutralizer Solution 100 mL USD 22.47
S0125 EpiLife® Defined Growth Supplement (EDGS) 5 mL USD 47.00
S0015 Human Keratinocyte Growth Supplement (HKGS) 5 mL USD 41.00
S001K Human Keratinocyte Growth Supplement Kit (HKGS Kit) 1 kit USD 52.00
S0175 Supplement S7 5 mL USD 65.00
R011K Coating Matrix Kit Protein 1 kit USD 44.50
17005042 Keratinocyte-SFM (1X) 500 mL USD 86.00
10744019 Defined Keratinocyte-SFM (1X) 500 mL USD 81.00