Ordering Information

Sku Name Size Price Qty
N7745100 Gibco® Rat Primary Cortical Astrocytes 1 mL USD 428.00

GIBCO® Rat Primary Astrocytes

  • Each vial contains 1 x 106 viable cells isolated from the cortices of Sprague-Dawley rats at embryonic day 19 (E19)
  • Superior cell morphology
  • Better lot-to-lot consistency

Superior cell viability

GIBCO® Rat Primary Cortical Astrocytes give consistent post-thaw viability in the range of 70–90%, while a competitor’s cell viability was only 50% post-thaw (Figure 1).

 Figure 1. Viability was determined by trypan blue exclusion using the Countess™ Cell Counter. GIBCO® Rat Primary Cortical Astrocyte viability is more than 80%, while astrocyte viability from a competitor is about 50%.

Superior proliferation potential

GIBCO® Rat Primary Cortical Astrocytes are cryopreserved at the end of passage 1 (P1), and can be recovered and further expanded for at least 2 additional passages (Figure 2).

 Figure 2. Rat Cortical Astrocytes were thawed and cultured in astrocyte medium containing 85% high glucose DMEM and 15% fetal bovine serum. Cells were passaged every eight days by dissociation and replating on a plastic culture vessel.

Phenotypic marker expression of GIBCO® Rat Primary Cortical Astrocytes

GIBCO® Rat Primary Cortical Astrocytes stain positive for astrocyte-specific marker glial fibrillary acidic protein (GFAP) upon expansion. Figure 3 shows more than 80% of cells are positive for GFAP and less than 10% of the cells stain positive for neuron and oligodendrocyte markers.

Figure 3.
Fluorescence images (20X) of Rat Cortical Astrocytes at P2 cultured for 3 days and stained for the appropriate phenotypic markers. Nuclei were stained with DAPI (blue). While approximately 80% of the cells stain positive for the astrocyte marker GFAP (A), less than 10% of the cells are positive for immature neuronal marker doublecortin (DCX) (B)  and oligodentrocyte marker galactosylceramide (GalC) (C).