Puromycin is an aminonucleoside antibiotic produced by the bacterium Streptomyces alboniger. It inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation. It is a potent translational inhibitor in both prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces. Puromycin has a fast mode of action, causing rapid cell death at low antibiotic concentrations. Adherent mammalian cells are sensitive to concentrations of 2 to 5 µg/ml, while cells in suspension are sensitive to concentrations as low as 0.5 to 2 µg/ml. Puromycin-resistant stable mammalian cell lines can be generated in less than one week.

Ordering Information

Sku Name Size Price Qty
A1113802 Puromycin Dihydrochloride 20 mL USD 407.00
A1113803 Puromycin Dihydrochloride 10 x 1 mL USD 215.00

Table 1 - Suggested Puromycin Concentration

Cell Line Concentration [µg/ml] Reference
HT1080 (human fibrosarcoma)0.2–0.4
1
2
J Biol Chem 282:29314–29322 (2007)
J Virol 82:3320–3328 (2008)
Cancer Res 68: 1417–1426 (2008)
HeLa (human cervical cancer)1-2
2
2
Genes Cells12:397–406 (2007)
Mol Cell Biol 28:4104–4115 (2008)
PNAS 105:16490–16495 (2008)
HEK293 (human embryonic kidney)0.25
2
3
Nucleic Acids Res 36:e83 (2008)
Mol Cell Biol 28:4104–4115 (2008)
Mol Cell Biol 27:4708–4719 (2007)
Hep G2 (human hepatocellular carcinoma)0.3
2
2
J Biol Chem 283:21462–21468 (2008)
Mol Cell Biol 28:4104–4115 (2008)
J Biol Chem 283:708–715 (2008)
Human embryonic stem (ES) cells0.5
1
5
Nucleic Acids Res 36:e148 (2008)
Stem Cells 26:850–863 (2008)
Stem Cells 26:2245–2256 (2008)
MCF-7 (human breast cancer)0.45
1
2
RNA 13:1375-1383 (2007)
Cancer Res 68:1319–1328 (2008)
Mol Cell Biol 28:4104–4115 (2008)
H1299 (non-small cell lung carcinoma)1
1.5
2.5
Nucleic Acids Res 35:e17 (2007)
J Biol Chem 283:33394–33405 (2008)
Proc Natl Acad Sci USA 105:1937–1942 (2008)
MDA-MB-231 (human breast cancer)0.5
1.5
5
Mol Cell Biol 28:997–1006 (2008)
Mol Cell Biol 27:324 – 339 (2007)
Mol Cell Biol 28:687–704 (2008)
A549 (lung cancer)1.5
1.5
Mol Cell Biol 27:324–339 (2007)
J Biol Chem 283:33394–33405 (2008)