The design and performance of the patented Microporator MP-100 electronic pipette transfection chamber was described by Kim et al.. The inventors described that the innovative design caused a remarkable increase in cell viability and transfection efficency compared to traditional cuvette based electroporation systems. The Microporation MP-100 has helped many researchers improve their transfection experiments. As a result, there are now more than 40 scientific publications that cite the successful use of the Microporator MP-100 for delivery of plasmid DNA and siRNA.
Kim JA, Cho K, Shin MS, et al. (2008) A novel electroporation method using a capillary and wire-type electrode. Biosens Bioelectron 23(9):1353–1360.
Invitrogen developed the Neon® Transfection System from the Microporator MP-100 platform, by updating the touch screen user interface, pre-programmed gradient optimization procedure, and generating additional cell type specific protocol database of cell-specific protocols. The Neon® Transfection system kits (with buffers and Neon® Pipette Tips) are fully compatible with the original Microporator MP-100.