Neon® Transfection System
High transfection efficiency and high cell viability in a broad range of cell lines
Figure 1. High transfection efficiency of Jurkat cells with Neon® Tranfection System.
Intracellular uptake of reporter vector encoded with EGFP at 24 hr following transfection of Jurkat cells with Neon® Transfection System. B is the corresponding fluorescence image of A.
Examples of cell lines successfully transfected with the Neon® Transfection System
|Cell line||Cell type||Transfection efficiency (%)*||Viable cells (%)|
|Primary rat cortical cells
|Primary rat hippocampal cells||Brain, hippocampal
Protocols and reference information for a large number of cell lines are available in Neon® cell database
Neon® Pipette Tip design vs. standard electroporation cuvette
* Kim JA, Cho K, Shin MS, et al. (2008) A novel electroporation method using a capillary and wire-type electrode. Biosens Bioelectron 23(9):1353-1360.
Figure 2. Standard electroporation cuvette compared to Neon® pipette tip.
The design of the electrode in pipette has been shown to produce a more uniform electric field. The result is less toxicity to the cells and higher transfection efficiencies.
Plug-n-play out of the box
- Load mix of harvested cells and molecules to be delivered (e.g., DNA, RNA, protein) into the Neon™ Pipette tip.
- Plug the pipette into position in the Neon® transfection device; select your protocol, and press Start.
- Unplug the pipette and transfer your transfected cells into a tissue culture vessel.
Simplified transfection kit for all cell types
Transfection couldn’t be simpler, into any cell type.