When selecting a transfection method, consider the payloadyou wish to deliver (DNA, RNA, or protein) and the type of cellsyou want to transfect. Use the tables below to choose between the various cationic-lipid transfection reagents and the Neon® Transfection System available from Life Technologies™.
We offer options for DNA, siRNA, oligonucleotide, and RNA delivery, giving you more confidence in your results.
- Superior delivery—transfect a broad range of cell types, including those notoriously difficult to transfect, with high efficiency
- Nontoxic performance—you see results due to the presence of your nucleic acid, not the reagent
- Less optimization—rapid, simple protocols provided for most cell lines
Transfection reagent selection guide
Continuous cell lines are capable of unlimited proliferative potential, and are generally easier to work with than primary or finite cell cultures. However, because these cells have undergone genetic transformation to become immortalized, their behavior in culture may not necessarily reflect the in vivo situation.
|Easy-to-transfect adherent cells||Hard-to-transfect adherent cells||Suspension cells|
|NEW Lipofectamine® MessengerMAX™|
|Invivofectamine® 2.0||In vivo delivery to liver following tail vein injection|
Primary cells are isolated directly from the tissue and proliferated under appropriate conditions. As such, they are morphologically and physiologically more similar to an in vivo state. However, they are usually more difficult to culture and transfect than continuous cell lines.
After the first subculture, the primary culture becomes known as a cell line. Cell lines derived from primary cultures have a limited life span (i.e., they are finite), and as they are passaged, cells with the highest growth capacity predominate, resulting in a degree of genotypic and phenotypic uniformity in the population. Therefore, their phenotype is intermediate between primary cells and continuous cultures. The use of such cells is sometimes easier than the use of primary cells, especially for the generation of stably transfected clones.
|DNA for expression of protein, shRNA, and miRNA||mRNA for expression of protein|
|Non-coding RNA for RNAi inhibition of gene expression||Co-delivery for cotransfection of
RNAi vectors and siRNAs
Additional Life Technologies™ transfection reagents
|Transfection reagent||Key features & applications of reagent|
|ExpiFectamine® 293 Transfection Kit||
|FreeStyle® MAX Transfection Reagent||
|Lipofectamine® 2000 CD Transfection Reagent||
|Oligofectamine® Transfection Reagent||
|293fectin™ Transfection Reagent||
|Cellfectin® II Transfection Reagent||
|DMRIE-C Transfection Reagent||
|Lipofectamine® Transfection Reagent||
|Lipofectin® Transfection Reagent||
|Optifect™ Transfection Reagent||