Note: Our new Lipofectamine® Reagent Protocols have been newly optimized for efficiency, viability, and reproducibility across a broad range of cell types.  This is often the best place to start, especially in a new cell line.  If you find this doesn’t work for your specific cell type, then you can you look to our cell specific protocols below for further optimization.

Cell Type Source Application Recommended Transfection Reagent Recommended Transfection SKU Protocol/
Reference
0 - 9
10T1/2 Mouse embryonic fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015 PDF
293-F  Human kidney Protein Production 293fectin™ 12347-019 PDF
293T Human embryonic kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150 Page
3T3L1 Mouse fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
3T3L1 Mouse fibroblast siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
786-O Human renal cell adenocarcinoma cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
A-F
A549 Human lung carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
A549 Human lung carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
ACHN Human renal cancer  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
ARPE-19 Human retina Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
B16-F10 Mouse melanoma Plasmid DNA Lipofectamine® 18324-012 18324-020   Page
BAEC Bovine aortic endothelial cells  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
BGOV-1 Human embryonic stem cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
BHK-21 Hamster kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Breast Adipose Fibroblasts Human breast adipose siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
C2C12 Mouse muscle myoblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
C3A Human hepatocellular Carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
C6 Rat glioma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Caco-2 Human colorectal adenocarcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Cardiac Fibroblasts Adult rat heart siRNA Lipofectamine®  RNAiMAX 13778-075 13778-150   Page
CaSki Human cervical carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
CHO K1 Hamster ovary Plasmid DNA Lipofectamine® LTX  15338-100 15338-500   PDF
CHO-S  Hamster ovary Protein Production FreeStyle™ MAX Reagent 16447-100 16447-500   PDF
CMMT Rhesus monkey mammary. tumor  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Cortical Neurons, E18 Rat brain Plasmid DNA Lipofectamine® LTX  15338-100 15338-500   Page
COS 7L Monkey kidney fibroblast siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
COS 7L Monkey kidney fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
CV-1 Primate kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
CV-1 Primate kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
D3 Mouse embryonic stem cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
D3 Mouse embryonic stem cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Daoy Human medulloblastoma cell line Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
DL-3 Mouse T lymphoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
DLD-1 Human colon adenocarcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
DT40  Chicken Bursa siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
EOC Human epithelial ovarian cancer  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Foreskin Fibroblast, Primary Human foreskin siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
G - H
Glial Cell Mouse brain siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
GripTite™ 293 MSR Human kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HaCaT Human immortalized keratinocytes Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HASMC Human aortic smooth muscle cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HCT116 Human colon carcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HCT116 Human colon carcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HEK 293 Human embryonic kidney Co-transfect Plasmid DNA and siRNA Lipofectamine® 3000 L3000008 L3000015   PDF
HEK 293 Human embryonic kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HEK 293 Human embryonic kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HeLa S3 Human cervical carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HeLa S3 Human cervical carcinoma Co-transfect Plasmid DNA and siRNA Lipofectamine® 3000 L3000008 L3000015   PDF
HeLa S3 Human cervical carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
Hepatocytes, Primary Mouse liver  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
HepG2 Human liver carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HepG2 Human liver carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Hippocampal Neurons Rat brain siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Hippocampal Neurons Rat brain Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HL60 Human lymphoblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HMEC-1 Human dermal microvascular endothelial cells Plasmid DNA Lipofectamine® LTX  15338-100 15338-500   PDF
HMVEC-L Human lung microvascular endothelial cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HNSCC Human head and neck squamous cell carcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
HT 29 Human colon adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HT1080 Human fibrosarcoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HT1080 Human fibrosarcoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HT1376 Human grade III transitional bladder cell carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Huh-7 Human hepatocellular carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Huh-7 Human hepatocellular carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Human Mesenchymal Stem Cells Human bone marrow siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
Human Mesenchymal Stem Cells Human bone marrow Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HUVEC Human umbilical vein cord siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HUVEC Human umbilical vein cord Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
I - M
IMR-90 Human embryonic lung siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
IMR-90 Human embryonic lung Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
INS-1 Rat pancreatic {beta}-cell line siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
JEG-3 Human choriocarcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Jurkat Human T-cell leukemia Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
JygMC(A) Mouse mammary carcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
K562 Human myelogenous leukemia siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
K562 Human myelogenous leukemia Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
L929 Mouse fibroblast cell line Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
LC-NCI-H23 Non-small cell, epid. Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
LNCap Human prostate cancer Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
LS180 Human colon carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
LSMC Human leiomyoma smooth muscle cells  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
M21 Human melanoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
McA-RH7777 Rat hepatoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
MCF 7 Human breast cancer siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
MCF 7 Human breast cancer Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MCF 7 Human breast cancer Co-transfect Plasmid DNA and siRNA Lipofectamine® 3000 L3000008 L3000015   PDF
MDA-MB-231 Human breast adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MDA-MB-435 Human breast adenocarcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
MDA-MB-435 Human breast adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MDCK Madin-Darby canine kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MDCK Madin-Darby canine kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
MDM Human monocyte-derived macrophages  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
ME-180 Human cervical carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
MEF Mouse embryonal fibroblast cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Mesangial Cells, Primary Rat kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
mIMCD3 Murine inner medullary collecting duct  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
MIN6 Mouse pancreatic {beta}-cell line siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
MLT Mouse Leydig tumor siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Molt-4 Human T lymphoblast  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MRC-5 Human lung  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
N - R
N2a-APP  Mouse neuroblastoma N2a cells stably expressing human APP695  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
N-38 Mouse hypothalamic cell line siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NB  Human neuroblastoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NBS Human Nijmegen breakage syndrome cells  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NCI-H460 Human large cell lung cancer  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
NDHF  Neonatal dermal fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Neonatal Rat Ventricular Cardiomyocyte Rat heart muscle siRNA Lipofectamine® RNAiMAX 13778-075 13778-150    
Neural Progenitor Cells, Primary Embryonic mouse brain  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Neuro2A Mouse brain neuroblastoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Neuro2A Mouse brain neuroblastoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NG108-15 Hybrid of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1 cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
NHFF Human diploid somatic fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
NIH 3T3 Mouse embryonic fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
NIH 3T3 Mouse embryonic fibroblast siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
NOR-10 (H-2b) Mouse skeletal muscle cell line siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NRK Rat normal kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
NT2 Human teratocarcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NTera2 Human embryonic carcinoma stem cell  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
OLN-93, Primary Rat brain glial cultures siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
OLP, Primary Rat cerebra siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
P19 Mouse embryonal carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
P19 Mouse embryonal carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
PC12 Rat neuronal Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
PC3 Human prostate adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Rat1 Rat fibroblast cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
RAW264.7  Mouse macrophage cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
RPC-C2A  Rat dental pulp cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
S - W
S2-013 Human pancreas Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Saos-2 Human osteosarcoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Sf9  Insect ovary DNA Cellfectin® 10362-010     Page
SK-BR3 Human breast carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SK-MEL-28 Human malignant melanoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SK-N-SH Human neuroblastoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SK-N-SH Human neuroblastoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
SK-OV-3 Human ovarian adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SNB19 Human glioblastoma cell line Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SW1417 Human colon adenocarcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
SW480 Human colon adenocarcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
T47D Human mammary adenocarcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
T98G Human brain siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
THP-1 Human monocytic leukemia Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
THP-1 Human monocytic leukemia siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
U251 Human glial Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
U2OS Human osteosarcoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
U87 Human astrocyte Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Vero African green monkey kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
WI-38(VA13) Normal human fetal lung fibroblast  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page