Note: Our new Lipofectamine® Reagent Protocols have been newly optimized for efficiency, viability, and reproducibility across a broad range of cell types.  This is often the best place to start, especially in a new cell line.  If you find this doesn’t work for your specific cell type, then you can look to our cell-specific protocols below for further optimization.

Cell Type Source Application Recommended Transfection Reagent Recommended Transfection SKU Protocol/
Reference
0 - 9
10T1/2 Mouse embryonic fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
293-F  Human kidney Protein Production 293fectin™ 12347-019     PDF
293T Human embryonic kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
3T3L1 Mouse fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
3T3L1 Mouse fibroblast siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
786-O Human renal cell adenocarcinoma cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
A-F
A549 Human lung carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
A549 Human lung carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
ACHN Human renal cancer  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
ARPE-19 Human retina Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
B16-F10 Mouse melanoma Plasmid DNA Lipofectamine® 18324-012 18324-020   Page
BAEC Bovine aortic endothelial cells  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
BGOV-1 Human embryonic stem cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
BHK-21 Hamster kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Breast Adipose Fibroblasts Human breast adipose siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
C2C12 Mouse muscle myoblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
C3A Human hepatocellular Carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
C6 Rat glioma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Caco-2 Human colorectal adenocarcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Cardiac Fibroblasts Adult rat heart siRNA Lipofectamine®  RNAiMAX 13778-075 13778-150   Page
CaSki Human cervical carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
CHO K1 Hamster ovary Plasmid DNA Lipofectamine® LTX  15338-100 15338-500   PDF
CHO-S  Hamster ovary Protein Production FreeStyle™ MAX Reagent 16447-100 16447-500   PDF
CMMT Rhesus monkey mammary. tumor  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Cortical Neurons, E18 Rat brain Plasmid DNA Lipofectamine® LTX  15338-100 15338-500   Page
COS 7L Monkey kidney fibroblast siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
COS 7L Monkey kidney fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
CV-1 Primate kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
CV-1 Primate kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
D3 Mouse embryonic stem cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
D3 Mouse embryonic stem cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Daoy Human medulloblastoma cell line Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
DL-3 Mouse T lymphoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
DLD-1 Human colon adenocarcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
DT40  Chicken Bursa siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
EOC Human epithelial ovarian cancer  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Foreskin Fibroblast, Primary Human foreskin siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
G - H
Glial Cell Mouse brain siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
GripTite™ 293 MSR Human kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HaCaT Human immortalized keratinocytes Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HASMC Human aortic smooth muscle cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HCT116 Human colon carcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HCT116 Human colon carcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HEK 293 Human embryonic kidney Co-transfect Plasmid DNA and siRNA Lipofectamine® 3000 L3000008 L3000015   PDF
HEK 293 Human embryonic kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HEK 293 Human embryonic kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HeLa S3 Human cervical carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HeLa S3 Human cervical carcinoma Co-transfect Plasmid DNA and siRNA Lipofectamine® 3000 L3000008 L3000015   PDF
HeLa S3 Human cervical carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
Hepatocytes, Primary Mouse liver  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
HepG2 Human liver carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HepG2 Human liver carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Hippocampal Neurons Rat brain siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Hippocampal Neurons Rat brain Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HL60 Human lymphoblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HMEC-1 Human dermal microvascular endothelial cells Plasmid DNA Lipofectamine® LTX  15338-100 15338-500   PDF
HMVEC-L Human lung microvascular endothelial cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HNSCC Human head and neck squamous cell carcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
HT 29 Human colon adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HT1080 Human fibrosarcoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HT1080 Human fibrosarcoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HT1376 Human grade III transitional bladder cell carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Huh-7 Human hepatocellular carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Huh-7 Human hepatocellular carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Human Mesenchymal Stem Cells Human bone marrow siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
Human Mesenchymal Stem Cells Human bone marrow Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
HUVEC Human umbilical vein cord siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
HUVEC Human umbilical vein cord Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
I - M
IMR-90 Human embryonic lung siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
IMR-90 Human embryonic lung Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
INS-1 Rat pancreatic {beta}-cell line siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
JEG-3 Human choriocarcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Jurkat Human T-cell leukemia Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
JygMC(A) Mouse mammary carcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
K562 Human myelogenous leukemia siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
K562 Human myelogenous leukemia Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
L929 Mouse fibroblast cell line Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
LC-NCI-H23 Non-small cell, epid. Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
LNCap Human prostate cancer Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
LS180 Human colon carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
LSMC Human leiomyoma smooth muscle cells  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
M21 Human melanoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
McA-RH7777 Rat hepatoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
MCF 7 Human breast cancer siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
MCF 7 Human breast cancer Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MCF 7 Human breast cancer Co-transfect Plasmid DNA and siRNA Lipofectamine® 3000 L3000008 L3000015   PDF
MDA-MB-231 Human breast adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MDA-MB-435 Human breast adenocarcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
MDA-MB-435 Human breast adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MDCK Madin-Darby canine kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MDCK Madin-Darby canine kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
MDM Human monocyte-derived macrophages  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
ME-180 Human cervical carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
MEF Mouse embryonal fibroblast cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Mesangial Cells, Primary Rat kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
mIMCD3 Murine inner medullary collecting duct  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
MIN6 Mouse pancreatic {beta}-cell line siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
MLT Mouse Leydig tumor siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
Molt-4 Human T lymphoblast  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
MRC-5 Human lung  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
N - R
N2a-APP  Mouse neuroblastoma N2a cells stably expressing human APP695  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
N-38 Mouse hypothalamic cell line siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NB  Human neuroblastoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NBS Human Nijmegen breakage syndrome cells  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NCI-H460 Human large cell lung cancer  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
NDHF  Neonatal dermal fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Neonatal Rat Ventricular Cardiomyocyte Rat heart muscle siRNA Lipofectamine® RNAiMAX 13778-075 13778-150    
Neural Progenitor Cells, Primary Embryonic mouse brain  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Neuro2A Mouse brain neuroblastoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Neuro2A Mouse brain neuroblastoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NG108-15 Hybrid of mouse neuroblastoma N18TG2 and rat glioma C6-BU-1 cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
NHFF Human diploid somatic fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
NIH 3T3 Mouse embryonic fibroblast Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
NIH 3T3 Mouse embryonic fibroblast siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
NOR-10 (H-2b) Mouse skeletal muscle cell line siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NRK Rat normal kidney siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
NT2 Human teratocarcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
NTera2 Human embryonic carcinoma stem cell  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
OLN-93, Primary Rat brain glial cultures siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
OLP, Primary Rat cerebra siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
P19 Mouse embryonal carcinoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
P19 Mouse embryonal carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
PC12 Rat neuronal Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
PC3 Human prostate adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Rat1 Rat fibroblast cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
RAW264.7  Mouse macrophage cells Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
RPC-C2A  Rat dental pulp cells siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
S - W
S2-013 Human pancreas Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Saos-2 Human osteosarcoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Sf9  Insect ovary DNA Cellfectin® 10362-010     Page
SK-BR3 Human breast carcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SK-MEL-28 Human malignant melanoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SK-N-SH Human neuroblastoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SK-N-SH Human neuroblastoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   PDF
SK-OV-3 Human ovarian adenocarcinoma  Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SNB19 Human glioblastoma cell line Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
SW1417 Human colon adenocarcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
SW480 Human colon adenocarcinoma Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
T47D Human mammary adenocarcinoma  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
T98G Human brain siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
THP-1 Human monocytic leukemia Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
THP-1 Human monocytic leukemia siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
U251 Human glial Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
U2OS Human osteosarcoma siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page
U87 Human astrocyte Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
Vero African green monkey kidney Plasmid DNA Lipofectamine® 3000 L3000008 L3000015   PDF
WI-38(VA13) Normal human fetal lung fibroblast  siRNA Lipofectamine® RNAiMAX 13778-075 13778-150   Page