Revolutionizing the field of genome editing

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Pushing the boundaries of science in critical disciplines

July Feature Story

Revolutionizing the field of genome editing

Recent advancements in molecular and cell biology have enabled researchers with simple yet powerful genome editing tools like the Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) system—making it easier to target and regulate user-defined endogenous genes in a sequence-specific manner. With CRISPR, researchers can create mouse models of human diseases much more quickly than before, study individual genes much faster, and easily change multiple genes in a cell at once to study their interactions. They have the potential to be applied in large-scale cell engineering applications across broad cell types, thereby accelerating research in the field of gene therapy and other applied markets.

Empowering large-scale cell engineering

The CRISPR-Cas9 system is a new approach to genome editing that provides rapid, efficient editing with multiplexing capabilities. With their highly flexible but specific targeting, CRISPR-Cas9 systems can be manipulated and redirected to become powerful tools for genome editing. From the developers of GeneArt® Precision TALEN™ technologies, we bring you a suite of CRISPR-Cas9 products and services to help accelerate your gene editing. For monitoring the efficiency of your genome editing experiments, we’ve developed the GeneArt® Genomic Cleavage Detection Kit, which provides a simple, reliable, and rapid method to determine nuclease cleavage efficiency at a given locus.

Featured products:

GeneArt® CRISPR Nuclease Vectors

The GeneArt® CRISPR Nuclease Vector Kits are a simple, ready-to-use, all-in-one expression vector system using both a Cas9 nuclease expression cassette and a guide RNA cloning cassette for rapid and efficient cloning of a target-specific crRNA. This system allows you to edit and engineer the genomic locus of your choice in a sequence-specific manner from a single plasmid. Two different reporters—OFP (Orange Fluorescent Protein) and CD4—are available to allow for flow-cytometry based sorting or bead-based enrichment of edited cell population and tracking of transfection efficiency.

The GeneArt® CRISPR Nuclease Vector system offers:

  • An easy-to-design genome engineering system
  • Affordable, ready-to-use cloning vectors
  • Enrichment for cell lines hard to transfect or difficult to modify

GeneArt® CRISPR Nuclease mRNA

The GeneArt® CRISPR Nuclease mRNA is wild type Cas9 as mRNA for genome editing with CRISPR-Cas9 technology. Cas9 mRNA is ready to transfect and circumvents the need for time-consuming cloning steps required when using CRISPR vector systems. Cas9 mRNA is co-transfected with a custom GeneArt® CRISPR Strings™ DNA fragment encoding a target-specific guide RNA (gRNA), which directs the Cas9 protein to the intended genome locus to create a double-stranded break. Additionally, the Cas9 mRNA can be used in multiplexing approaches with more than one GeneArt® CRISPR Strings™ fragment.

The GeneArt® CRISPR Nuclease mRNA lets you:

  • Circumvent time-consuming cloning steps
  • Screen several CRISPR guide RNA sequences at once
  • Avoid promoter constraints when used with T7-based CRISPR Strings™ fragments

Application note: Improve your genome editing outcomes

Lipofectamine® 3000 reagent was developed to break through the boundaries of delivery and facilitate new technologies, such as genome engineering, in more biologically relevant systems. With this new reagent, GeneArt® Precision TALEN™ and CRISPR constructs show improved transfection efficiency. These advancements in delivery help minimize painstaking downstream workflows, enable easier stem cell manipulation, and enhance site-specific insertion of transgenes into the cellular genome. Download now

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