Get started with cloning

We offer powerful and versatile Invitrogen™ cloning and expression vectors, GeneArt® gene synthesis and assembly tools, and molecular biology essentials for that critical first step in your experiment. Unsure where to start? Which cloning method is right for me?

An introduction to cloning

Most popular cloning products

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Cartoon of synthetic DNA
Cartoon of engineered chromosome

Featured cloning categories

5-minute, room-temperature cloning with no gel purification or post-PCR modifications & up to 95% recombinants with TOPO® cloning

High-efficiency cloning of blunt-ends using Zero Blunt® cloning kits.

Easily clone fragments using TOPO XL® & GeneArt® Seamless Cloning technology

TA cloning technology relies on the complimentary of thymine & adenine. Enhanced with ExpressLink™ T4 DNA Ligase.

Choose from a range of restriction & modifying enzymes & new, fast ligase.

A variety of scales, formats, modifications, & purifications to meet your cloning needs..

Industry-leading sequence analysis & data management software.

Combining the convenience of the Invitrogen™ online ordering system, with cutting-edge GeneArt® service offerings

Based on Type IIs restriction enzymes for non-recombination-based cloning of multiple DNA inserts.

Gateway® recombination cloning technology allows gene shuttling, enabling you to access virtually any expression system.

Choose competent cells based on application, strain, packaging format & transformation efficiency.

PCR enzymes for any application: high fidelity, high specificity, long fragment, GC rich, genotyping, or routine PCR

Clone up to 10 DNA fragments simultaneously; assemble existing fragments without restriction digest or PCR amplification.

Cloning support center
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Cloning resources

NEW Cloning Support Center
Find tips, troubleshooting help, and resources for your cloning applications

Tools

We offer everything from custom vector design to high-throughput protein expression.

Recorded seminars